000 01763cab a2200289Ia 45 0
999 _c47761
_d47761
001 u194502
003 SIRSI
008 110822s2011 ua ss b eng d
040 _aEAL
041 _aeng
_bara
090 _aART AVMJ V57 No128 23
100 1 _aDarwish,Samah.F.
_92639
240 1 0 _aAssiut veterinary medical journal, 2011 v. 57 (128)
_h[electronic resource].
245 1 0 _aEvalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma
_h[electronic resource].
246 1 5 _aتقيم اختبار تفاعل البلمرة المتسلسل التقليدى مقارنة باختبار تفاعل البلمرة المتسلسل الكمى للكشف عن فيروس الهربس البقرى1- فى كل من السائل المنوى وبلازما السائل المنوى البقرى.
300 _ap.285-299.
504 _aIncludes references.
520 _aThe aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays.
546 _aSummary in Arabic.
650 0 _aCows
_xDiseases.
_92640
650 0 _aSemen
_xAnalysis.
_92641
650 0 _aCattle
_xHealth.
_92642
700 1 _aAmin,A.S.
_92643
773 0 _tAssiut Veterinary Medical Journal.
_g2011.v.57(128)
_x1012-5973
_7nnas
_wu181356
856 4 0 _uhttp://nile.enal.sci.eg/EALE/2011/AVMJ/5711/128/285.pdf
_zFull Text Article.
942 _cAR
_2lcc