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001 | u194502 | ||
003 | SIRSI | ||
008 | 110822s2011 ua ss b eng d | ||
040 | _aEAL | ||
041 |
_aeng _bara |
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090 | _aART AVMJ V57 No128 23 | ||
100 | 1 |
_aDarwish,Samah.F. _92639 |
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240 | 1 | 0 |
_aAssiut veterinary medical journal, 2011 v. 57 (128) _h[electronic resource]. |
245 | 1 | 0 |
_aEvalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma _h[electronic resource]. |
246 | 1 | 5 | _aتقيم اختبار تفاعل البلمرة المتسلسل التقليدى مقارنة باختبار تفاعل البلمرة المتسلسل الكمى للكشف عن فيروس الهربس البقرى1- فى كل من السائل المنوى وبلازما السائل المنوى البقرى. |
300 | _ap.285-299. | ||
504 | _aIncludes references. | ||
520 | _aThe aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays. | ||
546 | _aSummary in Arabic. | ||
650 | 0 |
_aCows _xDiseases. _92640 |
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650 | 0 |
_aSemen _xAnalysis. _92641 |
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650 | 0 |
_aCattle _xHealth. _92642 |
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700 | 1 |
_aAmin,A.S. _92643 |
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773 | 0 |
_tAssiut Veterinary Medical Journal. _g2011.v.57(128) _x1012-5973 _7nnas _wu181356 |
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856 | 4 | 0 |
_uhttp://nile.enal.sci.eg/EALE/2011/AVMJ/5711/128/285.pdf _zFull Text Article. |
942 |
_cAR _2lcc |