| 000 | 03314cab a2200289Ia 45 0 | ||
|---|---|---|---|
| 001 | u197531 | ||
| 003 | SIRSI | ||
| 008 | 120103s2005 ua ss b eng d | ||
| 040 | _aEAL | ||
| 041 |
_aeng _bara |
||
| 090 | _aART AJARX V50 No2 3 | ||
| 100 | 1 | _aDowidar,Y. A. | |
| 240 | 1 | 0 |
_aAlexandria journal of agricultural research, 2005 v. 50 (2) _h[electronic resource]. |
| 245 | 1 | 0 |
_aEffects of seasonal variations on physical, molecular and freezability of friesian and buffalo bull semen _h[electronic resource]. |
| 246 | 1 | 5 | _aتأثير التغيرات الموسمية علي الصفات الطبيعية و الجزيئية و القابلية للتجميد للسائل المنوي لطلائق الفريزيان و الجاموس. |
| 300 | _aP. 15-21. | ||
| 504 | _aIncludes references. | ||
| 520 | _aSeven mature Friesian and seven mature buffalo bulls. proven to be free of venereal and infectious diseases. were subjected to twice per week semen collection by an artificial vagina throughout four consecutive Seasons (Year 2002-2003). Ejaculates were initially assessed for physical characteristics and high quality specimens were pooled within a species. processed and frozen. Individual sperm motility percentages were recorded after dilution. equilibration and thawing. Additionally an aliquot of seminal plasma representing species within a season was subjected to sodium dodecyl sulphate electrophoresis (SDS - PAGE) to monitor the changes in type and intensity of seminal plasma peptides. Mean ejaculate volume was higher (P<0.05) in Friesian (5.1ml) than in buffalo (3.5ml) bulls. However, season has no significant effect on ejaculate volume. Sperm cell concentration and percent progressive motility were higher (P<0.05) for both buffalo and Friesian bulls during winter and autumn than during summer. Percent of post thaw survival was lowest during summer (48.3%) as compared with that obtained in winter (66.75%~). spring (65.4%) or autumn (61.7%). Percent of post thaw sperm surviva1 was higher (P<0.05) for Friesian (64.6%) than buffalo (56.6%) bulls. Number of peptide bands in seminal plasma of Friesian bulls were higher (16.15.15 and 17) than in buffalo (10.11. 12and 12)for seminal plasma of winter. spring. autumn and .summer. respectively. There appears to exist one or two more peptide hands in summer than in winter seminal plasma. A darker band with molecular weight of 17.9 kDa was found in Friesian. but not in buffalo seminal plasma. Moreover. there are two more peptide bands (Mwt. 23.4 and 32 kDa) found in Friesian seminal plasma which were absent in buffalo. On the contrary. there existed a band of 14 kDa in buffalo. but not in Friesian seminal plasma. this study concluded that climatic season has a significant influence on the physical and chemical characteristics of semen and, in turn. affecting its freezability and subsequent fertility. keywords season Friesian, buffalo sperm,freezing, peptide pattern. | ||
| 546 | _aSummary in Arabic. | ||
| 650 | 0 | _aFriesian cattle. | |
| 650 | 0 | _aFrozen semen. | |
| 650 | 0 | _aSemen. | |
| 650 | 0 | _aWater buffalo. | |
| 773 | 0 |
_tAlexandria Journal of Agricultural Research. _g2005.v.50(2) _x0044-250 _7nnas _wu158486 |
|
| 856 | 4 | 0 |
_uhttp://nile.enal.sci.eg/EALE/2005/AJARX/5005/2/15.pdf _zFull text Article. |
| 596 | _a1 | ||
| 942 |
_cAR _2lcc |
||
| 999 |
_c50502 _d50502 |
||