000			02682cab a2200325Ia 45 0
999			_c52098 _d52098
001			u199398
003			SIRSI
008			101222s2004 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART AVMJ V50 No101 9
100	1		_aMontasser, A. M. _91838
240	1	0	_aAssiut veterinary medical journal, 2004 v. 50 (101) _h[electronic resource].
245	1	0	_aApplication of in situ hybridization in diagnosis of brucellosis in tissues of naturally infected sheep and goats _h[electronic resource].
246	1	5	_aتطبيق التهجين الموضعي في تشخيص مرض البروسيلا في أنسجة الأغنام و الماعز المصابة طبيعيا بالمرض.‪
300			_ap.110-125.
504			_aIncludes references.
520			_aBrucellosis in sheep and goats are very common in Egypt and can be diagnosed by bacteriological and serological techniques. The present study was carried out for the assessment of sensitivity and specificity of in situ hybridization (ISH). One hundred animals (50 sheep and 50 goats) from an area with history of brucella infection were included and examined serologically by Buffered acidified plate test (BAP AT) Rose Bengal plate test (RBPT), Serum agglutination test (SAT), rivanol test and complement fixation test (CFT). Out of them 19 animals were reactors serologically by one or more test (10 from sheep and 9 from goats). From 19 reactors animals, proper samples from spleen and lymph nodes were collected for bacteriological, histopathological examination and ISH techniques. The results obtained from isolation trails, 7 brucella isolets were isolated (4 from sheep and 3 from goats). The isolated straines typed as Brucella melitensis biovar 3. The results obtained by ISH on paraffin embedded section of lymph nodes using a digoxigenin labeled DNA fragment located at gene encoding at 31 KDa Brucella abortus protein, revealed 12 cases out of 19 reactors animals were positive lymph nodes. The sensitivity of ISH techniques was higher than that of microbial culture where as the specificity of ISH was 100% when compared with the conventional microbial culture. Key words: Hybridization, Brucellosis, Sheep, Goats.
546			_aSummary in Arabic.
650		0	_aSheep _xDiseases. _91839
650		0	_aGoats _xDiseases. _91840
650		0	_aHybridization. _91841
650		0	_aBrucellosis in goats. _9379
650		0	_aBrucellosis in sheep. _9379
700	1		_aHelmy, Nashwa M. _9496
700	1		_aAhmed, Hanem M. _91842
773	0		_tAssiut Veterinary Medical Journal. _g2004.v.50(101) _x1012-5973 _7nnas _wu181356
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2004/AVMJ/5004/101/110.pdf _zFull Text Article.
942			_cAR _2lcc