| 000 | 02428cab a2200277Ia 45 0 | ||
|---|---|---|---|
| 999 |
_c52109 _d52109 |
||
| 001 | u199411 | ||
| 003 | SIRSI | ||
| 008 | 120411s2004 ua ss b eng d | ||
| 040 | _aEAL | ||
| 041 |
_aeng _bara |
||
| 090 | _aART AVMJ V50 No103 7 | ||
| 100 | 1 |
_aShalaby, H. A. _9382 |
|
| 240 | 1 | 0 |
_aAssiut veterinary medical journal, 2004 v. 50 (103) _h[electronic resource]. |
| 245 | 1 | 0 |
_aSnail derived antigens useful in diagnosis of fascioliasis _h[electronic resource]. |
| 246 | 1 | 5 | _aمولدات ضد من القوقع لها أهمية فى تشخيص الإصابة بالديدان الكبدية. |
| 300 | _ap.80-93. | ||
| 504 | _aIncludes references. | ||
| 520 | _aL. cailliaudi feet and hepatopancreases antigens which would be of use as a specific immunodiagnostic test for fascioliasis were evaluated and characterized using ELISA and Western blot techniques. ELISA tests demonstrated the successful detection of F. gigantica IgG antibodies in naturally infected animals using both antigens. But, L. cailliaudi hepatopancreases antigen showed higher sensitivity in antibody detection than L. cailliaudi feet. SDS-PAGE provided evidence of shared antigens between F. gigantica ES, L. cailliaudi feet and L. cailliaudi hepatopancreases antigens. Those observations were extended by demonstrating the value of the antigenic epitopes of L. cailliaudi hepatopancreases in detection of anti-Fasciola antibodies in naturally infected animals using western blot technique. Where, from several polypeptides showed specific reactivity toward F gigantica infected animal sera, the 64 KDa polypeptide was common with F gigantica ES antigen and was not detected in L. cailliaudi feet antigen. This polypeptide might be reinforced the sensitivity of L. cailliaudi hepatopancreases antigen to be higher than that of L. cailliaudi feet antigen. These results suggested that L. cailliaudi hepatopancreases constituted a rich source of antigen for serodiagnostic studies of F. gigaritica. Besides, it was easy to obtain and with a low cost compared to other Fasciola antigens as ES, crude worm and egg. | ||
| 546 | _aSummary in Arabic. | ||
| 650 | 0 |
_aFascioliasis _xDiagnosis. _9501 |
|
| 650 | 0 |
_aSnails _xControl. _91083 |
|
| 650 | 3 |
_ahepatopancreas. _91829 |
|
| 773 | 0 |
_tAssiut Veterinary Medical Journal. _g2004.v.50(103) _x1012-5973 _7nnas _wu181356 |
|
| 856 | 4 | 0 |
_uhttp://nile.enal.sci.eg/EALE/2004/AVMJ/5004/103/80.pdf _zFull Text Article. |
| 942 |
_cAR _2lcc |
||