000			04284cab a2200277Ia 45 0
001			u200521
003			SIRSI
008			110710s2011 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART EJPB V15 No4 8
100	1		_aAbd El-Gelil, Hanan M.
240	1	0	_aEgyptian journal of plant breeding, 2011 v. 15 (4) _h[electronic resource].
245	1	0	_aUtilization of morphological and molecular markers to evaluate a new genotype resistant to some insect attacks in Egyptian cotton _h[electronic resource].
246	1	5	_aاستخدام الدلائل المورفولوجية و الجزئيية لتقييم تركيب وراثي جديد من القطن المصري المقاوم لبعض الآفات.
300			_ap.105-124.
504			_aIncludes references.
520			_aField experiments were conducted during 2007 and 2008 seasons in EI-Giza Experimental station, A.R. C to eval1late a new nectariless cotton genotype, namely Giza 85 Ne which descended from discontinuous backcrossing between the Egyptian cotton variety Giza 85 and a nectariless Upland One, viz., MAR (NLG8CDGP6H-I-95). It was compared with their parents, Le., Giza 85 and the nectariless line. The Experimental design was a randomized complete block with three repUcations. Only the Egyptian parent plots were sprayed against insect infestations. Comparative genetic studies among evaluated of genotypes were done using morphological characters related. to insect resistance, yield components, fiber quality as well as a histological comparisons among the three evaluated entries. Besides a molecular genetic fingerprinting using RAPD analysis was done. The obtained results can be summarized as follows: Field and morphological characterization of the new hybrid Giza 85 Ne revealed that nectary was not found on the midrib on the lower side of each leaf and below the bracts when compared with the Egyptian cotton variety Giza 85. The results indicated that insignificant differences were found between Giza 85 Ne. and Giza 85 for boll weight, seed cotton yield, lint yield, ant percentage, seed index, micronaire and fiber length. By Contrast, significant differences were reported for Pressley. Besides, leaf anatomy for the thlck1iess of cuticle showed that the upper and lower epidermis were increased in both the nectariless parental line (Ne) and the new hybrid Giza 85 Ne compared with the local variety Giza 85. The relative resistance of the new cotton genotype to insects was associated with thicker leaf. In the RAPD analysis, ten primers were used and succeeded in the identification and differentiation of the three genotypes. Moreover, accession - specific DNA markers characterized different genotypes and therefore were used to generate unique fingerprint for each genotype. The total number of amplicons was 117 DNA fragments whlle, ninety bands (76.9 ") were polymorphic amplicons. There were some unique bands in the new hybrid which were not observed in the parental genotypes. With respect to the genetic similarities among the new hybrid Giza 85 Ne and the two parents, the highest al1rtIlarity. was 90.1 " between the hybrid and the Egyptian cotton parent. While, the least similarity was observed between the two parents (53.9 "). It should be noted, however that there were still some level of polymorphlsms between the hybrid Giza 85 Ne and the Egyptian cotton variety Giza 85. Thus, more backcrosses should be done to the Egyptian parent in order. to restore the Egyptian genomic background, while, maintaining the new characters for inset resistance. From the above results, it could be concluded that the new genotype Giza 85 Ne had high yield/d and yield components, showed desirable fiber quality as well as good level of insect resistance. For that the breeder can use this genotype as good material for insect resistance in the cotton breeding program.
546			_aSummary in Arabic.
650		0	_aSea Island cotton _xGenetics.
650		0	_aSea Island cotton _xMorphology.
650		0	_aSea Island cotton _xDiseases and pests _xControl.
773	0		_tEgyptian Journal of Plant Breeding. _g2011.v.15(4) _x1110-7863 _7nnas _wu191048
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2011/EJPB/1511/4/105.pdf _zFull Text Article.
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