000 02370cab a2200337Ia 45 0
001 u203038
003 SIRSI
008 121106s2011 ua ss b eng d
040 _aEAL
041 _aeng
_bara
090 _aART ZVJ V39 NO3 14
100 1 _aAmmar, AM
240 1 0 _aZagazig veterinary journal, 2011 v. 39 (3)
_h[electronic resource].
245 1 0 _aAdvanced studies on diagnosis of single M. gallisepticum infection and combined with E. coli in chickens
_h[electronic resource].
246 1 5 _aدراسات متقدمة على تشخيص العدوى الأحادية بالميكو بلازما جاليسيبتكم والمقترنة بالميكروب القولونى فى الدواجن.‪
300 _ap.124-136.
504 _aIncludes references.
520 _aMycoplasma gallisepticum (MG) is a persistent, highly transmissible chicken pathogen. It predisposes the birds to other infections such as Escherichia coli yielding significant losses in performance and associated economics to all sectors of the poultry industry. This study aims to evaluate the role of MG and E. coli virulence genes in occurrence of respiratory diseases in chickensusing advanced techniques. It was noted that MG and/or E. coli were accused of CRD in naturally infected chickens. PPLO medium, Digitonin test and biochemical reactions were used for characterization of Mycoplasma while E. coli was identified through isolation on MacConkey's and EMB agar media, biochemical tests and rapid API 20E. Serotyping of E. coli isolates showed that 078 was the most predominant serotype (33.33%) followed by 027 (22.22%), other serotypes as 086a, 0115, 029, 0125 and 0146 were also included. Conventional PCR on MG isolates showed the presence of 16S rRNA and mgc2 genes with characteristic bands at 330 and 824 bp, respectively.
546 _aSummary in Arabic.
650 0 _aMycoplasma gallisepticum.
650 0 _aMycoplasma diseases
_xDiagnosis.
650 0 _aChickens
_xDiseases.
650 0 _aEscherichia coli infections in animals.
650 0 _aPolymerase chain reaction
_xDiagnostic use.
700 1 _aEissa, S 1
700 1 _aYousreya, HM
700 1 _aAbd El Aziz, EE
773 0 _tZagazig Veterinary Journal.
_g2011.v.39(3)
_x1110-1458
_7nass
_wu189860
856 4 0 _uhttp://nile.enal.sci.eg/EALE/2011/ZVJ/3911/3/124.pdf
_zFull Text Article.
596 _a1
942 _cAR
_2lcc
999 _c54849
_d54849