| 000 | 01555cab a2200241Ia 45 0 | ||
|---|---|---|---|
| 001 | u206868 | ||
| 003 | SIRSI | ||
| 008 | 110417s2001 ua ss b eng d | ||
| 040 | _aEAL | ||
| 090 | _aART AJVS V17 No1 1 | ||
| 100 | 1 | _aTemsah, S. | |
| 240 | 1 | 0 |
_aAlexandria journal of veterinary science, 2001 v. 17 (1) _h[electronic reource]. |
| 245 | 1 | 0 |
_aProduction, purification, characterization and immunological studies of E.coli E-U₁₀ penicillin amidase _h[electronic reource]. |
| 300 | _ap.1-16. | ||
| 504 | _aIncludes references. | ||
| 520 | _aPenicillin amidase enzyme (PA) was isolated from the culture filtrate of one isolated strain of E. coli E-U₁₀ grown on optimized medium after 24 hours incubation. The enzyme was purified to homogeneity by sequential ammonium sulfate fractionation and ion exchange and gel filtration chromatography. On using Disc-PAGE, the final purification fraction showed only one distinctive band indicating high purity. On using SDS-PAGE the final fraction showed two distinctive units with molecular weight of 22000 and 59000 Dalton. The optimal pH, temperature and time course for activity were 7.5, 37°C and 10 minutes respectively. The enzyme with penicillin G as substrate had a Km of 13 mM and Vmax 3.92 U/ml. | ||
| 650 | 0 | _aEscherichia coli. | |
| 653 | _apenicillin amidase. | ||
| 700 | 1 | _aMabrouk, A. | |
| 773 | 0 |
_tAlexandria Journal of Veterinary Science. _g2001.v.17(1) _x1110-2047 _7nnas _wu191343 |
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| 856 | 4 | 0 |
_uhttp://nile.enal.sci.eg/EALE/2001/AJVS/1701/1/1.pdf _zFull Text Article. |
| 596 | _a1 | ||
| 942 |
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_c57052 _d57052 |
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