000			02165cab a2200337Ia 45 0
001			u208761
003			SIRSI
008			150217s2002 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART AJB V5 No1 1
100	1		_aIbrahim, M.A.
240	1	0	_aArab journal of biotechnology, 2002 v. 5 (1) _h[electronic resource]: _b an international journal devoted to the advancement of biotechnology.
245	1	0	_aGenetically modified potato (Solanum tuberosum L.) resistant to potato tuber moth (Phthorimaea operculella) _h[electronic resource
246	1	5	_aنباتات بطاطس معدلة وراثيا لمقاومة فراشة درنات البطاطس
300			_ap.1-10
504			_aIncludes references.
520			_aPotato tuber moth (PTM), Phthorimaea operculella, causes tremendous economic losses to potato production in Egypt. An Egyptian isolate of the entomopathogenic bacteria Bacillus thuringiensis (Bt), isolate CI2, produces a potent Cry1Aa7 toxin that kills the larval stages of PTM, more efficiently than other standard Bt toxins. The cry1Aa7 gene cloned in the binary vector pBI121 and pBI-cry7 construct was transformed to Agrobacterium tumefaciens strain LBA4404 viaBtriparental mating. Transformed LBA4404 strains harboring pB1-cry7 were used to mobilize the cry1Aa7 cassette into the potato cultivar Desiree. Out of 91 transformants, five individual lines (D8, D9, D13, D26 and D37) were shown to be transgenic for cry1Aa7. The integrated toxin gene as well as the expressed Cry1Aa7 was confirmed using polymerase chain reaction (PCR) and enzyme-linked immunosorbant assay (ELISA), respectively.
546			_aSummary in Arabic.
650		0	_aPotatoes _xGenetics
650		0	_aPotatoes _xBreeding
650		0	_aPotatoes _xDiseases and pest resistance
650		0	_aPotato tuberworm _xBiological control
700	1		_aMetry, E.A.
700	1		_aOsman, Y.A.
700	1		_aNasr El-Din, T.M.
700	1		_aMadkour, M.A.
773	0		_tArab Journal of Biotechnology. _g2002.v.5(1) _x1110-6875 _7nnas _wu189256
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2002/AJB/502/1/1.pdf _zFull Text Article
596			_a1
942			_cAR _2lcc
999			_c58470 _d58470