000			02123cab a2200301Ia 45 0
999			_c60007 _d60007
001			u210822
003			SIRSI
008			101222s2014 ua ss b eng d
040			_aEAL
041			_aeng _bara
043			_af-ua
090			_aART AVMJ V60 No141 14
100	1		_aAttia, E. R. H. _9459
240	1	0	_aAssiut veterinary medical journal, 2014 v. 60 (141) _h[electronic resource].
245	1	0	_aPrevalence of leptospirosis in Egyptian sheep flock _h[electronic resource].
246	1	5	_aانتشار مرض الليبتوسبيرا فى قطيع أغنام فى مصر.
300			_ap.105-111.
504			_aIncludes references.
520			_aA sheep flock contains 600 head of Al-Barky ewes suffered from: Icterus, bloody urine, death two days post symptoms appearance and abortion in 6 pregnant ewes. The symptoms appeared on 46 case and 6 ewes aborted and mortality rate was44 case dead. The results of examined samples showed that the isolation of leptospira from 5 out of 52 (9.62 %) animals samples (blood, urine and necropsy kidney tissue samples), the isolates were 2 from blood samples (6.7 %), 2 from kidney samples (6.7 %) and 1 from urine samples (10 %). The obtained leptospira isolates from infected ewes were examined by PCR for identification of leptospires using universal primers set (lig1/lig2,) that amplifY the genomic DNA of all pathogenic Leptospiraserovar only, the most predominant seropositivity using MAT was detected against L.int.icterohaemorrhagiae (57.69%), followed by L.int.grippotyphosa (28.85%) and L.int.pomona (13.46%). The highest titer of seropositivity was detected against serovarL.int.icterohaemorrhagiae (1: 1600) while L. int.grippotyphosa and L. int.pomona reached up to ( 1:400).
546			_aSummary in Arabic.
650		0	_aSheep _xDiseases _zEgypt. _923
650		0	_aLeptospirosis in animals _xPrevention. _9460
700	1		_aDohrieg, R. M. A. _9497
700	1		_aEl-Shafei, M. A. _9498
773	0		_tAssiut Veterinary Medical Journal. _g2014.v.60(141) _x1012-5973 _7nnas _wu181356
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2014/AVMJ/6014/141/105.pdf _zFull Text Article.
942			_cAR _2lcc