000			03029cab a2200385Ia 45 0
999			_c61126 _d61126
001			u212353
003			SIRSI
008			160811s2014 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART AVMJ V60 No143 9
100	1		_aHamed, Maha I. _9246
240	1	0	_aAssiut veterinary medical journal, 2014 v. 60 (143) _h[electronic resource].
245	1	0	_aITS2 region-based molecular identification of fungal pathogens in equine corneal ulceration _h[electronic resource].
246	1	5	_aالتححديد الجزيئى القائم على المنطقة ITS2 للفطريات الممرضة المسببة لتقرح القرنية فى الخيول.
300			_ap.61-69.
504			_aIncludes references.
520			_aThis study aimed to assess the utility of polymerase chain reaction (PCR) and DNA sequencing in diagnosing fungal ulcerative keratitis in horses and compare their sensitivity with conventional microbiologic techniques used in laboratories. Conjunctival swabs from 12 horses with corneal ulcerations admitted to the Veterinary Teaching Hospital, College of Veterinary Medicine, Purdue University were collected for examinations. 14 conjunctival swabs were analyzed by the conventional culture method and by generic PCR using universal fungal primers to amplify the internal transcribed spacer 2 (ITS2) genetic region followed by DNA sequencing. The conventional culture method revealed that two samples exhibited fungal growth that identified as Aspergillus sp. and Penicillium sp. while three conjunctival samples contained bacterial growth which was identified as Staphylococcus intermedius, Staphylococcus epidermidis and Streptococcus zooepidemicus. Interestingly, PCR followed by DNA sequencing of the biological specimens on the swabs identified fungal pathogens in 9/14 samples and Ascomycete species in 3/14 samples. In 2/14 samples, no fungal pathogen was identified using PCR. Fungal pathogens detected included Aspergillus sp. in six eyes, Penicillium sp. in one eye, Fusarium sp. in one eye and Cladosporium sp. in one eye. Our findings demonstrate a limitation of the conventional culture method as a diagnostic tool as a fungal pathogen was detected in only two samples by this method as compared to nine samples using PCR and DNA sequencing. Key words: ITS2 region, fungal pathogens, equine, corneal ulcers.
546			_aSummary in Arabic.
650		0	_aCornea _xUlcers. _9247
650		0	_aCorneal diseases _9248
650		0	_aHorses _xDiseases _xTreatment. _9249
650		0	_aPathogenic fungi _9213
650		0	_aPolymerase chain reaction. _9250
650		0	_aDNA _xTherapeutic use. _9251
650		3	_ainternal transcribed spacersg _9252
650		3	_aopacity _9253
653			_acorneal ulceration
700	1		_aMohammad, Haroon. _9254
700	1		_aTownsend, Wendy. _9255
700	1		_aSeleem, Mohamed N. _9256
773	0		_tAssiut Veterinary Medical Journal. _g2014.v.60(143) _x1012-5973 _7nnas _wu181356
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2014/AVMJ/6014/143/61.pdf _zFull Text Article.
942			_cAR _2lcc