000 cab a22 a 4500
999 _c64117
_d64117
001 u193882
003 ENAL
008 170403s2017 ua ss b eng d
040 _aEAL
_cEAL
041 _aeng
_bara
090 _aART ASEJ V38 No1 4
100 1 _aBondok, Abdel Aziz. T.
_924913
240 1 0 _aAlexandria science exchange journal, 2017 v. 38 (1)
_h[electronic resource]:
245 1 0 _aEvaluation of breeding programs susceptibility for two important forage crops using DNA barcoding
_h[electronic resource]
246 1 5 _aتقييم قابلية نوعين من محاصيل العلف لبرامج التربية باستخدام تقنية DNA Barcoding
300 _aP. 28-37
504 _aIncludes references.
520 _aThis investigation was carried out to identify and evaluate Medicago sativa and Trifolium alexandrinum probability for breeding program based on two bar-coding genes (rbcl and Cox1 genes). Identification of Medicago sativa Baladi 1 was performed through rbcl and Cox1 genes. Medicago sativa Baladi 1 was identified as Medicago sativa voucher G00199095 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast (Sequence ID: KJ204375.1) and Medicago sativa voucher Ahrendsen_23 for rbcl and Cox1 genes respectively. Identity values were recorded with 90% of identity for alfalfa, Baladi 1 Genotype ribulose – 1/5 – bisphosphate carboxylase / oxygenase large subunit (rbcl) gene (sequences ID: KJ206375.1) also, identity values were recorded with 91.24% of identity for alfalfa Baladi 1 Genotype, cytochrome c oxiddase bubunit I gene (cox 1) (sequence ID: KJ 204375.1). Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) as rbcl and Cox1 genes respectively. Affiliation of genetic origin was detected for Trifolium alexandrinum with 100 % of similarity with origin source which indicate highly possibility for applying breeding programs comparing with Medicago sativa which reflect the lowest genetic similarity with origin source.
546 _aSummary in Arabic.
650 0 _aForage plants
_xBreeding
_924914
650 2 _aDNA Barcoding, Taxonomic
_924915
700 _924916
773 0 _tAlexandria Science Exchange Journal.
_g2017.v.38(1)
_x1110-0176
_7nnas
_wu193445
856 4 0 _uhttp://nile.enal.sci.eg/EALE/2017/ASEJ/3817/1/27.pdf
_zFull Text Article
942 _cAR
_2alc