000			01903cab a2200265Ia 4500
001			u159600
003			SIRSI
008			100329s2019 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART EJBPC V29 No1 13
100	1		_aHassan, Mohamed M. _933677
240	1	0	_aEgyptian journal of biological pest control, 2019 v. 29 (1) _h[electronic resource]:
245	1	0	_aRapid identification of Trichoderma koningiopsis and Trichoderma longibrachiatum using sequence characterized amplified region markers _h[electronic resource].
300			_ap.93-100.
347			_atext file _bPDF
504			_aIncludes reference.
520			_aMolecular markers enable the detection and classification of fungi isolated from their natural environments. To develop species-specific markers for detecting Trichoderma koningiopsis and T. longibrachiatum, the sequence-characterized amplified region technique, using 20 inter-simple sequence repeat-polymerase chain reaction primers, was performed. The two specific markers for amplifying a single unique band consistent with T. koningiopsis and T. longibrachiatum, which were absent with other Trichoderma strains, were successfully identified. These fragments had no meaningful sequence homology with known sequences available in the National Center for Biotechnology Information and TrichOKEY databases. Compared with traditional identification techniques, these markers can facilitate more rapid and less complicated studies of Trichoderma population dynamics and evaluate their establishment after release into agricultural environments.
546			_aSummary in Arabic
700			_4Farid, Mona A.
700			_4Gaber,Ahmed
773	0		_tEgyptian Journal of Biological Pest Control. _g2019.v.29(1) _7nnas _wu159598
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2019/EJBPC/1929/1/13.pdf _zFull Text Article.
942			_cAR _2lcc
999			_c67445 _d67445