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| 001 | u159600 | ||
| 003 | SIRSI | ||
| 008 | 100329s2019 ua ss b eng d | ||
| 040 | _aEAL | ||
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_aeng _bara |
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| 090 | _aART EJBPC V29 No1 16 | ||
| 100 | 1 |
_aKorany, Shereen M. _918045 |
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| 240 | 1 | 0 |
_aEgyptian journal of biological pest control, 2019 v. 29 (1) _h[electronic resource]: |
| 245 | 1 | 0 |
_aEntomopathogenic efficacy of the chitinolytic bacteria: aeromonas hydrophila isolated from siwa oasis, egypt _h[electronic resource]. |
| 300 | _ap.111-120. | ||
| 347 |
_atext file _bPDF |
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| 504 | _aIncludes reference. | ||
| 520 | _aThirty-four bacterial isolates were isolated from soil samples collected from the North Western Coast and a water sample collected from brackish water at Siwa Oasis, Matrouh Governorate, Egypt. Only six isolates showed chitinase activity when screened on colloidal chitin agar medium. The highest chitinolytic activity was achieved by a bacterial isolate labeled as A.S. This isolate was identified as Aeromonas hydrophila based on analysis of 16S rRNA gene sequence and morphological, physiological, and biochemical characteristics. Optimization of the cultural conditions for maximum chitinase production by A. hydrophila revealed that the highest level of chitinase was recorded when the bacterium was grown in malt nitrogen-based medium containing 1% colloidal chitin at pH7 for 48 h incubation at 30 °C. Crude chitinase from isolate A. hydrophila was evaluated against first instar larvae of the greater wax moth; Galleria mellonella L. (Lepidoptera: Pyralidae) at different concentrations of 0, 185, 205, 235, 265, 295 U/mg protein. It increased larval and pupal mortality rates in a concentration-dependent manner. The tested crude chitinase significantly induced a decrease in adults’ emergence rate and their fecundity. | ||
| 546 | _aSummary in Arabic | ||
| 700 | _4Mansour, Amany N. | ||
| 700 | _4El-Hendawy, Hoda H. | ||
| 700 | _4Kobisi, Abdel Naser A. | ||
| 700 | _4Aly, Hamdy H. | ||
| 773 | 0 |
_tEgyptian Journal of Biological Pest Control. _g2019.v.29(1) _7nnas _wu159598 |
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| 856 | 4 | 0 |
_uhttp://nile.enal.sci.eg/EALE/2019/EJBPC/1929/1/16.pdf _zFull Text Article. |
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