000 02998cab a2200325 a 4500
001 u182843
003 EAL
008 080823s2022 ua ss b eng d
040 _aEAL
_cEAL
041 _aeng
_bara
090 _aART JAPFPSU V11 No1 8
100 1 _aOsama, Enas
_934725
240 1 0 _aJournal of Animal, Poultry & Fish Production Suez Canal University, 2022 v. 11 (1)
_h[electronic resource]
245 1 0 _aEffect of rabbit semen filtration methods on conception rates and altering sex ratio in artificially inseminated rabbits
_h[electronic resource]
246 1 5 _aتأثیر طرق ترشیح السائل المنوي على معدلات الحمل وتغییر النسبة الجنسیة في الأرانب الملقحة صناعیا
300 _a55 - 63 p.
347 _atext file
_bPDF
504 _aIncludes reference.
520 _aThe present study was conducted to study the effect of filtering rabbit semen through five different filters: Sephadex-G15, Albumin, Cotton, Synthetic Fiber, Sand and Sperm swim-up method on conception rate of artificially inseminated rabbit does. Also, attempting to separate X-and Y-chromosome bearing spermatozoa by testing three semen fractions from each filter. Three methods were used to perform semen examinations: the 1stmethod was CASA to examine the filtered semen motility parameters, the 2ndmethod was Hoechst 33342 dye to stain the chromosomes to recognize X-and Y-spermatozoa and the 3rdmethod was determining sex ratio of bunnies born after insemination of rabbit does with selected fractions of filtered semen. Ten Chinchilla bucks and seventy-nine multiparous New Zealand White rabbit does were used in this study. Results clearly showed that semen filtration process and the selected semen fractions increased (P<0.05) percentages of sperm progressive motility, Y chromosome bearing spermatozoa, conception rate and male sex ratio of bunnies obtained than that before filtration. Conclusively, Sand, Albumin gradient, Fiber, Sephadex and Swim-up methods proved to be effective in improving semen quality and enabling sperm sexing which were accepted by Hoechst 33342 stain, CASA data and AI determinations of bunnies born in rabbits. These designed filters would enable researchers in this field to work in safe, less cost and effective methods to manage artificial insemination and reproduction in rabbit farms. Keywords: Rabbits, semen filtration, semen sexing, artificial insemination
546 _aSummary in Arabic.
650 0 _aRabbits as laboratory animals
650 0 _aRabbits
_97346
_xArtificial insemination
700 1 _aEL-Azzazi, Fakhri E.
700 1 _aKhalil, Hassan A.
_9969
700 1 _aKishk, Walied H.
_934728
700 1 _aAyoub, Mostafa A.
_934727
773 0 _tJournal of Animal, Poultry & Fish Production Suez Canal University.
_g2022.v.11(1)
_x2636-2732
_7nnas
_wu68741
856 4 0 _uhttp://nile.enal.sci.eg/EALE/2022/JAPFPSU/1122/1/55.pdf
_zFull Text Article.
942 _cAR
_2alc
999 _c68749
_d68749