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Vaccination of dogs against Rhipicephalus sanguineus ticks using salivary gland antigen [electronic resource].

By:

Abdel Gawad A. M

Contributor(s):

El-Kelesh, Eman, A. M

Language: English Summary language: Arabic Description: p.167-179Other title:

تحصين الكلاب ضد قراد الريباسيفليس سنجوينس باستخدام مستضد الغدد اللحابية [Added title page title]

Uniform titles:

Proceedings of the 1st scientific congress [electronic resource]:

Subject(s):

LOC classification:

ART ESAMP No1 9

Online resources:

Full Text Article.

In: Proceedings of the 1st Scientific Congress:the role of veterinary medicine in animal, and poultry, and fish wealth development : Giza, Egypt 30 -31 October, 2006Summary: The present study aimed to induce experimental resistance in dogs against Rhipicephalus sanguineus ticks using salivary gland antigen. Four dogs were vaccinated by salivary gland antigen obtained from partially fed adult ticks then challenged with 10 pairs of unfed adult ticks on the 35th day. The fifth dog remained as a control inoculated with adjuvant only. The results of vaccination showed a high protection in terms of de¬creased the percentage of tick attachment and significant reduction in the weight of engorged female ticks. SDS-PAGE analysis of salivary gland antigen revealed 14 bands ranging from 119.7 to 38 KDa. Immunoblotting with hyper-immune sera against salivary gland antigen showed 4 polypep¬tides. In contrast only three bands were detected with naturally infested dog sera, Three common polypeptides were recognized in both sera with molecular weights 97, 75 and 38 KDa, but two of them (97 and 38 Kda) were strongly recognized with hyper immune sera and faintly in naturally infested sera. One band of 88 KDa was recognized by hyper-immune sera only. Therefore, tick salivary gland antigen seem to have promising poten¬tials in inducing resistance in dogs.

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The present study aimed to induce experimental resistance in dogs against Rhipicephalus sanguineus ticks using salivary gland antigen. Four dogs were vaccinated by salivary gland antigen obtained from partially fed adult ticks then challenged with 10 pairs of unfed adult ticks on the 35th day. The fifth dog remained as a control inoculated with adjuvant only. The results of vaccination showed a high protection in terms of de¬creased the percentage of tick attachment and significant reduction in the weight of engorged female ticks. SDS-PAGE analysis of salivary gland antigen revealed 14 bands ranging from 119.7 to 38 KDa. Immunoblotting with hyper-immune sera against salivary gland antigen showed 4 polypep¬tides. In contrast only three bands were detected with naturally infested dog sera, Three common polypeptides were recognized in both sera with molecular weights 97, 75 and 38 KDa, but two of them (97 and 38 Kda) were strongly recognized with hyper immune sera and faintly in naturally infested sera. One band of 88 KDa was recognized by hyper-immune sera only. Therefore, tick salivary gland antigen seem to have promising poten¬tials in inducing resistance in dogs.

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