Alteration in electrophoretic muscle protein and esterase isozyme as an effect of some heavy metals in two species of tilapia [electronic resource].

Includes references.

Two species of tilapia (Sarotherodon galilaeus and Tilapia zillii) were used as experimental fish and treated with two doses (2 and 4 ppm) of cadmium (Cd), copper (Cu) and zinc (Zn) heavy metals at 96 and 192 hrs as common environmental pollutions. The results of electrophoretic analysis of muscle protein and esterase isozyme in this investigation revealed that both Sarotherodon galilaeus and Tilapia zillii species were affected by the treatments with two doses (2 and 4 ppm) of each metal. Some bands (represent the gene expression) were susceptible to pollution and disappeared from the treated fish. Muscle protein in fish exposed to 4 or 2 ppm of used heavy metals showed variations in the intensities and numbers of bands. The degree of polymorphism was high in all treatments. The intensities of bands were decreased after treatments compared with control. The correlation coefficients in band numbers of Sarotherodon galilaeus between control and treated fish with 4 ppm were negative at 96 hrs in Cd, Cu & Zn and at 192 hrs in Cd & Zn, while positive correlation coefficient was recorded for fish treated with 4 ppm of Cu at 192 hrs. Tilapla zillii showed a new band in treatment with 4 ppm of Cd at 96 hrs. The correlation coefficient was positive in fish treated with 4 ppm of used heavy metals and control. Esterase isozyme showed high polymorphism in treated fish and some new bands appeared. There was decrease in band numbers in some treatments in Tilapia zillii, while some new bands were appeared in Sarotherodon galilaeus.

Summary in Arabic.

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