Preliminary studies on the production of near isogenic lines resistant to blast disease via anther culture in rice [electronic resource].

Includes references.

The regenerated plantlets obtained from the F1 's via anther culture technique in rice, were adapted and grown in the greenhouse to produce seed. Twenty-five near isogenic lines succeeded to produce seeds. These lines were derived from two crosses namely Giza 159 x BL1 (18 lines) and Giza 159 x Pi 4 (7 lines). These near isogenic lines were grown with their respective parents for field evaluation for blast reaction as well as agronomic and yield characters. Three lines of the cross Giza 159 x BLl and one line of the cross Giza 159 x Pi 4 produced high yields comparable to their parents and exhibited resistance to blast. The entries at seedling stage were evaluated under greenhouse conditions using artificial inoculation by four identified pathotypes of the pathogen (Pyricularia oryza). All 18 lines of the cross Giza 159 X BLl were resistant to all isolates except one line which was moderately resistant to isolate no. 2. Meanwhile, out of seven lines of the cross Giza 159 x Pi 4, one and three lines were resistant to isolates no. land no.3, respectively. On other hand, one line was moderately resistant to isolate no. 2.The results showed also that Giza 159 was susceptible for all isolates except isolate no. 3, while both BLl and Pi 4 genotypes were resistant to all isolates. Thus, we can use both of them as donors to blast resistance in anther culture programs in rice. Further studies are needed to develop anther cultuer technique for increasing the number of regenerated plants to detect more near isogenic lines.

Summary in Arabic.

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