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Anther culture and plant regeneration in citrus (citrus volkameriana) [electronic resource].

By:

Aboshama, H. M. S

Language: English Summary language: Arabic Description: p.1717-1729Other title:

زراعة متوك الموالح وتكوين نباتات لأصل الفولكا [Added title page title]

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Mansoura University Journal of plant production, 2011 v. 2 (12) [electronic resource].

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In: Mansoura University Journal of Plant Production 2011.v.2(12)Summary: Development of an efficient and cost-effective doubled haploid production system In Citrus volkameriana is the prerequisite for the application of doubled haploid technology in a practical breeding program. An improvement of the protocol for haploid induction through anther culture of Citrus volkameriana was achieved following the evaluation of a number of the factors affecting androgenesis. Two types of media were tested for response of anther culture. In induction cultures, N6 Chu (1978) basal medium was more effective than Murashige and Skoog (1962). The influence of 2,4 dichlorophenoxcetic acid (2,4-0) and benzyladenirie (BA) and three temperature pre-treatments (4 C, 25°C, 3X'C) on the floral buds with respect to anther culture of C. volkameriana .

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Development of an efficient and cost-effective doubled haploid production system In Citrus volkameriana is the prerequisite for the application of doubled haploid technology in a practical breeding program. An improvement of the protocol for haploid induction through anther culture of Citrus volkameriana was achieved following the evaluation of a number of the factors affecting androgenesis. Two types of media were tested for response of anther culture. In induction cultures, N6 Chu (1978) basal medium was more effective than Murashige and Skoog (1962). The influence of 2,4 dichlorophenoxcetic acid (2,4-0) and benzyladenirie (BA) and three temperature pre-treatments (4 C, 25°C, 3X'C) on the floral buds with respect to anther culture of C. volkameriana .

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