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Production of new strawberry strains via anther culture [electronic resource]: 1- callus production, shoot formation and transplants production.

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Mohamed, Nagwa A

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Language: English Summary language: Arabic Description: p.841- 858Other title:

إنتاج بعض سلالات الفراولة الجديدة من خلال زرع المتوك: 1-إنتاج الكالس وتكوين الافرع وإنتاج الشتلات [Added title page title]

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Annals of agricultural science, 2002 v. 47 (3) [electronic resource].

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In: Annals of Agricultural Science 2002.v.47(3)Summary: This study was carried out at tbe Tissue Culture Lab. of Strawberry and Non- Traditional Crops Ceuter, Faculty of Agriculture, Ain Shams University from year 1998 to 2002 and completed at Zein El-Deen Strawberry Farm, Giza governorate. This study aimed to establisb an efficient protocol for generating somaclonal variations in sttawberry via antber culture and selecting tbe promising ones for tbe strawberry breeding programs in Egypt. Closed flower buds of Capitola, Chandler and Camarosa strawberry cultivars at the uninucleate stage directly following meiosis were used. Gresshofe and Doy (GD) and Damiano (D) media were tested for callus production while Linsmaier and Skoog (LS) & Murashige and Skoog (MS) media were tested for callus differentiation. Callus was regenerated into shoots which was placed on rooted medium. Then plantlets were acclimatized in plastic house. Transplants were planted in the nursery where runner formation, transplants production and quality were recorded. According to the yield performance (in the second paper), 10 strains from Camarosa, a strain from each of Capitola and Chandler were subjected to nursery trial in a randomized block design.

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This study was carried out at tbe Tissue Culture Lab. of Strawberry and Non- Traditional Crops Ceuter, Faculty of Agriculture, Ain Shams University from year 1998 to 2002 and completed at Zein El-Deen Strawberry Farm, Giza governorate. This study aimed to establisb an efficient protocol for generating somaclonal variations in sttawberry via antber culture and selecting tbe promising ones for tbe strawberry breeding programs in Egypt. Closed flower buds of Capitola, Chandler and Camarosa strawberry cultivars at the uninucleate stage directly following meiosis were used. Gresshofe and Doy (GD) and Damiano (D) media were tested for callus production while Linsmaier and Skoog (LS) & Murashige and Skoog (MS) media were tested for callus differentiation. Callus was regenerated into shoots which was placed on rooted medium. Then plantlets were acclimatized in plastic house. Transplants were planted in the nursery where runner formation, transplants production and quality were recorded. According to the yield performance (in the second paper), 10 strains from Camarosa, a strain from each of Capitola and Chandler were subjected to nursery trial in a randomized block design.

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