Biochemical and molecular genetic studies using SDS-protein, isozymes and RAPD-PCR in some common bean (Phaseolus vulgaris L.) cultivars [electronic resource].

Includes references.

SDS-protein and isozymes (esterase, glutamate oxaloacetate transminase, peroxidase and polyphenol oxidase) banding patterns and RAPD-PCR markers were used to genetically identify five Egyptian common bean (Phaseolus vulgaris L.) cultivars, which are grown in Egypt. The results showed a wide range of variation in total protein content, isozyme activities and SDS-protein banding patterns. However, low levels in the banding patterns of isozymes were observed. For RAPD-PCR analysis, seven random arbitrary primers were used. Twenty-five cultivar-specific markers (13 positive and 12 negative) were detected indicating that they can be used as markers for the five Phaseolus cultivars used in the present study. The phylogenetic relationships between the five common bean cultivars were determined by RAPD istance software package, version 1.04. Key words: Genetic fingerprinting, protein, isozymes, RAPD-PCR, Phaseolus.

Summary in Arabic.

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