Comparative studies between nitrogin fixing methylotrophic bacteria and rhizobia of some legume plants [electronic resource].

Includes references.

Ninety four isolates of methylotrophs were isolated from green leaves and roots of various legume plants. The ability of various Pink Pigmented Facultative Methylotrophic (PPFM) isolates to grow on Yeast Extract Mannitol (YEM) agar medium with or without 0.05% methanol as a source of carbon, some morphological, physiological characteristics and molecular biology studies, as well as biological nitrogen fixation activity by using 15N isotope technique , were estimated. The obtained results indicated that , PPFM.LI, PPFM.Sb and PPFM.D methylotrophic bacteria were isolated from Lupine, Soybean and handaqooq legume plants, respectevily, they had the ability to grow on YEM media with or without 0. 05% methanol and Congo red dye, as well as were able to induce nodule formation on roots of legume host plants. Morphological and cultural characteristics of the three PPFM isolates, as compared to reference rhizobial strains (USAD 110 and ARC 408), showed that ,all isolates were short rods , Gram negative and motile. Physiological characteristics of obtained PPFM isolates were catalase positive .All isolates could utilize sucrose and D-glucose, except PPFM.Lt, utilized citrate and methanol as a sole carbon, source except both rhizobial strains (USAD 110 and ARC 408).

Summary in Arabic.

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