Molecular characterization of new isolates of the entomopathogenic fungus beauveria bassiana and their efficacy against the tobacco caterpillar, spodoptera litura (fabricius) (lepidoptera: noctuidae) [electronic resource].

By: Contributor(s): Language: English Summary language: Arabic Description: p.60-68Uniform titles:
  • Egyptian journal of biological pest control, 2019 v. 29 (1) [electronic resource]:
Online resources: In: Egyptian Journal of Biological Pest Control 2019.v.29(1)Summary: Thirteen fungal isolates, having characteristics similar to Beauveria bassiana, were isolated from field soils of Punjab, India, on Beauveria-specific selective medium (BS medium). Molecular screening of the B. bassiana isolates, using PCR amplification with B. bassiana-specific primer and nucleotide sequence analysis of ITS region, confirmed only three isolates as B. bassiana (now named as BbR1, BbR2, and BbR3) along with MTCC 2028 reference isolate. Comparative RAPD-PCR amplification with ten different RAPD primers showed that all the three isolates were closely related
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Articles Articles Main ART EJBPC V29 No1 8 (Browse shelf(Opens below)) Available

Includes reference.

Thirteen fungal isolates, having characteristics similar to Beauveria bassiana, were isolated from field soils of Punjab,
India, on Beauveria-specific selective medium (BS medium). Molecular screening of the B. bassiana isolates, using PCR amplification with B. bassiana-specific primer and nucleotide sequence analysis of ITS region, confirmed only three isolates as B. bassiana (now named as BbR1, BbR2, and BbR3) along with MTCC 2028 reference isolate. Comparative RAPD-PCR amplification with ten different RAPD primers showed that all the three isolates were closely related

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