Evaluation of different levels of soybean lecithin as an alternative to egg yolk for cryopreservation of goat and ram spermatozoa
Khalifa, E. I.
Evaluation of different levels of soybean lecithin as an alternative to egg yolk for cryopreservation of goat and ram spermatozoa [electronic resource]. تقييم مستويات مختلفة من ليسيشين فول الصويا كبديل لصفار البيض لحفظ الحيوانات المنوية للماعز والكباش. - p.33-38
Includes references.
The purpose of the present study was to investigate the effect of different concentrations of soybean lecithin (SL) in goat and ram semen extenders as a substitute for egg yolk. In this study, thirty-six ejaculates were collected from each breed (3 adult goat bucks and 3 adult rams) aged >2.5 years old. Ejaculates were collected during the breeding season by means of an artificial vagina. The semen ejaculates were divided into six equal aliquots and diluted (1semen:4 diluent) to form six different cryoprotectant media (M). The control medium (MO) contained 2.5% and 15% egg yolk for goat bucks and ram semen extenders, respectively. Treatment extender media were M1 contained 1.5% SL, M2 included 2.5% SL, M3 supplemented with 3.5% SL, M4 contained 4.5% SL and M5 contained 5.5% SL. The semen samples were packed in straws (0.50 mL) and equilibrated in refrigeration at 5°C for 3 hours. After the equilibration period straws were warmed and sperm characteristics such as progressive motility, viability and abnormal tails were assayed. For freezing technique, the straws were suspended on a racK 5 cm above the liquid nitrogen vapor for 15 min at -120 °C. Then, straws immersed and stored in liquid nitrogen (-196°C). After thawing at 37°C/60 seconds, post- thawing motility, intact plasma membrane, sperm head damage and abnormal tails were evaluated. Results indicated that soybean lecithin at a level of 3.5 % attained higher (P < 0.05) sperm parameters than other concentrations of soybean lecithin and egg yolk in goat and ram semen extenders after the equilibration period.
Summary in Arabic.
Goats--Spermatozoa.
Goats--Germplasm resources--Cryopreservation.
Rams--Spermatozoa.
Rams--Germplasm resources--Cryopreservation.
Lecithin.
Frozen semen
Evaluation of different levels of soybean lecithin as an alternative to egg yolk for cryopreservation of goat and ram spermatozoa [electronic resource]. تقييم مستويات مختلفة من ليسيشين فول الصويا كبديل لصفار البيض لحفظ الحيوانات المنوية للماعز والكباش. - p.33-38
Includes references.
The purpose of the present study was to investigate the effect of different concentrations of soybean lecithin (SL) in goat and ram semen extenders as a substitute for egg yolk. In this study, thirty-six ejaculates were collected from each breed (3 adult goat bucks and 3 adult rams) aged >2.5 years old. Ejaculates were collected during the breeding season by means of an artificial vagina. The semen ejaculates were divided into six equal aliquots and diluted (1semen:4 diluent) to form six different cryoprotectant media (M). The control medium (MO) contained 2.5% and 15% egg yolk for goat bucks and ram semen extenders, respectively. Treatment extender media were M1 contained 1.5% SL, M2 included 2.5% SL, M3 supplemented with 3.5% SL, M4 contained 4.5% SL and M5 contained 5.5% SL. The semen samples were packed in straws (0.50 mL) and equilibrated in refrigeration at 5°C for 3 hours. After the equilibration period straws were warmed and sperm characteristics such as progressive motility, viability and abnormal tails were assayed. For freezing technique, the straws were suspended on a racK 5 cm above the liquid nitrogen vapor for 15 min at -120 °C. Then, straws immersed and stored in liquid nitrogen (-196°C). After thawing at 37°C/60 seconds, post- thawing motility, intact plasma membrane, sperm head damage and abnormal tails were evaluated. Results indicated that soybean lecithin at a level of 3.5 % attained higher (P < 0.05) sperm parameters than other concentrations of soybean lecithin and egg yolk in goat and ram semen extenders after the equilibration period.
Summary in Arabic.
Goats--Spermatozoa.
Goats--Germplasm resources--Cryopreservation.
Rams--Spermatozoa.
Rams--Germplasm resources--Cryopreservation.
Lecithin.
Frozen semen