Biochemical characterization of inhibin hormone in the ovary of she camel (Camelus dromedaries) (Record no. 37607)

MARC details
000 -LEADER
fixed length control field 02532cab a2200313Ia 45 0
001 - CONTROL NUMBER
control field u181361
003 - CONTROL NUMBER IDENTIFIER
control field SIRSI
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 100502s2007 ua ss b eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EAL
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
Language code of summary or abstract ara
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) ART AVMJ V53 No112 1
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Ramadan, A.A.
9 (RLIN) 102
240 10 - UNIFORM TITLE
Uniform title Assiut veterinary medical journal, 2007 v. 53 (112)
Medium [electronic resource].
245 10 - TITLE STATEMENT
Title Biochemical characterization of inhibin hormone in the ovary of she camel (Camelus dromedaries)
Medium [electronic resource].
246 15 - VARYING FORM OF TITLE
Title proper/short title التعرف على هرمون الانهبين في مبايض إناث الجمال باستخدام الطريقة الكيميائية الحديثة.
300 ## - PHYSICAL DESCRIPTION
Extent p.1-18
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes reference.
520 ## - SUMMARY, ETC.
Summary, etc. Isolation, purification and advanced characterization of hormone inhibin in ovary of female camel are aimed in this study. Pooled follicular fluid was collected from the ovaries ofshe-camel irrespective to physiological status and age of the animals. Follicular fluid was subjected to 2 types of gel filtration chromatography; Sephacryl S-200 where three peaks of proteins was obtained. The suspected peak to contain inhibin (peak III) resolved from S-200 was subjected to Sephadex 0-100 where three peaks were obtained, third peak was suspected to contain inhibin in pure form. To verifY the purity of the isolated hormone, the lyophilized fraction containing inhibin was subjected to analysis by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAOE) under both non reducing condition, stained with silver nitrate where a single band was resolved, and under reducing condition, where five bands resolved (ranging between 58.8 to 32.3 KDa). Using Reversed PhaseHigh Performance Liquid Chromatography (RP-HPLC) to fractionate the third peak, subunits of inhibin hormone eluted at different retention times. High Performance-Thin Layer Chromatography (HP-TLC) used to determine the N-terminal amino acids contents of the third peak and 7 amino acids resolved with different concentrations where histidine was the most abundant ofthe amino acids.
546 ## - LANGUAGE NOTE
Language note Summary in Arabic.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Inhibin
General subdivision Physiological effect.
9 (RLIN) 103
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Camels
General subdivision Diseases.
9 (RLIN) 65
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Camels
General subdivision Physiology
9 (RLIN) 65
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name El-Behairy, A.M.
9 (RLIN) 38
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Mostafa, M.A.
9 (RLIN) 104
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Al-Gabry, M.A.
9 (RLIN) 36
773 0# - HOST ITEM ENTRY
Title Assiut Veterinary Medical Journal.
Related parts 2007.v.53(112)
International Standard Serial Number 1012-5973
Control subfield nnas
Record control number u181356
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://nile.enal.sci.eg/EALE/2007/AVMJ/5307/112/1.pdf">http://nile.enal.sci.eg/EALE/2007/AVMJ/5307/112/1.pdf</a>
Public note Full Text Article.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Articles
Source of classification or shelving scheme Library of Congress Classification

No items available.


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