Biochemical characterization of inhibin hormone in the ovary of she camel (Camelus dromedaries) (Record no. 37607)
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000 -LEADER | |
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fixed length control field | 02532cab a2200313Ia 45 0 |
001 - CONTROL NUMBER | |
control field | u181361 |
003 - CONTROL NUMBER IDENTIFIER | |
control field | SIRSI |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 100502s2007 ua ss b eng d |
040 ## - CATALOGING SOURCE | |
Original cataloging agency | EAL |
041 ## - LANGUAGE CODE | |
Language code of text/sound track or separate title | eng |
Language code of summary or abstract | ara |
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AVMJ V53 No112 1 |
100 1# - MAIN ENTRY--PERSONAL NAME | |
Personal name | Ramadan, A.A. |
9 (RLIN) | 102 |
240 10 - UNIFORM TITLE | |
Uniform title | Assiut veterinary medical journal, 2007 v. 53 (112) |
Medium | [electronic resource]. |
245 10 - TITLE STATEMENT | |
Title | Biochemical characterization of inhibin hormone in the ovary of she camel (Camelus dromedaries) |
Medium | [electronic resource]. |
246 15 - VARYING FORM OF TITLE | |
Title proper/short title | التعرف على هرمون الانهبين في مبايض إناث الجمال باستخدام الطريقة الكيميائية الحديثة. |
300 ## - PHYSICAL DESCRIPTION | |
Extent | p.1-18 |
504 ## - BIBLIOGRAPHY, ETC. NOTE | |
Bibliography, etc. note | Includes reference. |
520 ## - SUMMARY, ETC. | |
Summary, etc. | Isolation, purification and advanced characterization of hormone inhibin in ovary of female camel are aimed in this study. Pooled follicular fluid was collected from the ovaries ofshe-camel irrespective to physiological status and age of the animals. Follicular fluid was subjected to 2 types of gel filtration chromatography; Sephacryl S-200 where three peaks of proteins was obtained. The suspected peak to contain inhibin (peak III) resolved from S-200 was subjected to Sephadex 0-100 where three peaks were obtained, third peak was suspected to contain inhibin in pure form. To verifY the purity of the isolated hormone, the lyophilized fraction containing inhibin was subjected to analysis by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAOE) under both non reducing condition, stained with silver nitrate where a single band was resolved, and under reducing condition, where five bands resolved (ranging between 58.8 to 32.3 KDa). Using Reversed PhaseHigh Performance Liquid Chromatography (RP-HPLC) to fractionate the third peak, subunits of inhibin hormone eluted at different retention times. High Performance-Thin Layer Chromatography (HP-TLC) used to determine the N-terminal amino acids contents of the third peak and 7 amino acids resolved with different concentrations where histidine was the most abundant ofthe amino acids. |
546 ## - LANGUAGE NOTE | |
Language note | Summary in Arabic. |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name entry element | Inhibin |
General subdivision | Physiological effect. |
9 (RLIN) | 103 |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name entry element | Camels |
General subdivision | Diseases. |
9 (RLIN) | 65 |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
Topical term or geographic name entry element | Camels |
General subdivision | Physiology |
9 (RLIN) | 65 |
700 1# - ADDED ENTRY--PERSONAL NAME | |
Personal name | El-Behairy, A.M. |
9 (RLIN) | 38 |
700 1# - ADDED ENTRY--PERSONAL NAME | |
Personal name | Mostafa, M.A. |
9 (RLIN) | 104 |
700 1# - ADDED ENTRY--PERSONAL NAME | |
Personal name | Al-Gabry, M.A. |
9 (RLIN) | 36 |
773 0# - HOST ITEM ENTRY | |
Title | Assiut Veterinary Medical Journal. |
Related parts | 2007.v.53(112) |
International Standard Serial Number | 1012-5973 |
Control subfield | nnas |
Record control number | u181356 |
856 40 - ELECTRONIC LOCATION AND ACCESS | |
Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2007/AVMJ/5307/112/1.pdf">http://nile.enal.sci.eg/EALE/2007/AVMJ/5307/112/1.pdf</a> |
Public note | Full Text Article. |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Koha item type | Articles |
Source of classification or shelving scheme | Library of Congress Classification |
No items available.