Polymerase chain reaction and adapted enzyme linked immunosorbent assay for diagnosis of camel brucellosis (Record no. 37794)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02054cab a2200277Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u181560 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 100510s2007 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART VMJ V55 No4 9 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Al-Dubaib, M. A. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Veterinary medical journal, 2007 v.55 (4) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Polymerase chain reaction and adapted enzyme linked immunosorbent assay for diagnosis of camel brucellosis |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تفاعل البوليميريز المتسلسل وأختبار الإلايزا غير المباشر المطوع لتشخيص الإصابة بالبروسيلا في الإبل. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.1067-1075 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes reference. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In the present study, polymerase chain reaction was carried out on 10 brucella isolates recovered from camels affected with brucellosis central region of Saudi Arabia. Brucella abortus as well as Brucella melitensis specific primers were employed for the assay. All isolates were identified as B. melitensis. This was in agreement with the results of the traditional bacteriological identification. Moreover, antibodies against camel IgG was raised in rabbits and purified with polystyrene affinity chromatography. The purified anti-camel IgG was conjugated with horseradish peroxidase (HRPO) using the sodium periodate method. The anticameJ-HRPO conjugate prepared in this study was tested in an indirect ELISA adapted in the same study on camel sera positive and negative for brucellosis as indicated by the Rose Bengal plate test. The conjugate was found efficient and was able to elucidate positive and negative samples at a dilution of 1/40. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Camels |
| General subdivision | Diseases. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Brucellosis in animals. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Polymerase chain reaction. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Veterinary Medical Journal. |
| Related parts | 2007.v.55(4) |
| International Standard Serial Number | 1110-1423 |
| Control subfield | nnas |
| Record control number | u159328 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2007/VMJ/5507/4/1067.pdf">http://nile.enal.sci.eg/EALE/2007/VMJ/5507/4/1067.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.