Micropropagation and genetic stability of Pyracantha fortuneana, roem. shrub (Record no. 41509)

MARC details
000 -LEADER
fixed length control field 02573cab a2200289Ia 45 0
001 - CONTROL NUMBER
control field u186259
003 - CONTROL NUMBER IDENTIFIER
control field SIRSI
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 101020s2009 ua ss b eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EAL
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
Language code of summary or abstract ara
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) ART EJH V36 No1 8
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name EI-Sharny, M. A.
240 10 - UNIFORM TITLE
Uniform title Egyptian journal of horticulture, 2009 v. 36 (1)
Medium [electronic resource].
245 10 - TITLE STATEMENT
Title Micropropagation and genetic stability of Pyracantha fortuneana, roem. shrub
Medium [electronic resource].
246 15 - VARYING FORM OF TITLE
Title proper/short title الاكثار الدقيق والثبات الوراثى لشجيرة البيراكانثا Pyracantha Fortuneana
300 ## - PHYSICAL DESCRIPTION
Extent p.149-161.
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes references.
520 ## - SUMMARY, ETC.
Summary, etc. This study was carried out in Plant Tissue Culture Laboratory at EI-Zohria Botanical Garden, Horticulture Research Institute, Agriculture Research Center during 2007-2008 seasons. The object of this study wa:; to establish a protocol for micropropagation and RAPD analysis for comparing between mother plant and in vitro plant of Pyracantha fortuneana Roem. The data revealed that for sterilization explant, using 40% c1orox for 25 min gave the best result (100% survival) and free contamination explant. Bud break and shoot development were best on full MS establishment medium with (2.0 mgll IBA) which gave the longest shootlet. While the greatest number of leaves was recorded on full MS-medium with (4.0 mglIBA). For shooting behaviour. the medium supplemented with (3.0 mgll BA) for four subcultures gave the greatest number of shootlet. But adding (4.0 or 5.0 mgtl kin) for four subcultures produced the tallest shoodet length. For the number of leaves. culture the explant on MS-medium have (4.0 mgt/ BA) for four subcultures gave the highest number of leaves. Regarding to the rooting behaviour, culturing the shootlet on MS-medium supplemented with (4.0 mg/lBA) produced the highest percentage of rooting, tallest root length and highest number of root. RAPD-based DNA fingerprints gave no evidence of somaclonal variation during the in vitro propagation protocol used. The RAPD profiles of tissue culture-derived plantlets revealed high similarity to mother tree. For acclimatization. the survival percentage was 70%.
546 ## - LANGUAGE NOTE
Language note Summary in Arabic.
596 ## -
-- 1
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Pyracantha.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Pyracantha
General subdivision Micropropagation.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sayed, Sawsan S.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Gomaa, S. A. A.
773 0# - HOST ITEM ENTRY
Title Egyptian journal of horticulture.
Related parts 2009.v.36(1)
International Standard Serial Number 1110-0206
Control subfield nnas
Record control number u183584
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://nile.enal.sci.eg/EALE/2009/EJH/3609/1/149.pdf">http://nile.enal.sci.eg/EALE/2009/EJH/3609/1/149.pdf</a>
Public note Full Text Article.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Articles
Source of classification or shelving scheme Library of Congress Classification

No items available.


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