Biological, serological and molecular studies on Prunus necrotic ring spot virus infecting Rosa hybrida L. in Egypt (Record no. 44124)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02509cab a2200325Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u189397 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110127s2008 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AJB V11 No1 12 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Abdel-Salam, Aly M. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Arab journal of biotechnology, 2008 v. 11 (1) |
| Medium | [electronic resource]: |
| -- | an international journal devoted to the advancement of biotechnology. |
| 245 10 - TITLE STATEMENT | |
| Title | Biological, serological and molecular studies on Prunus necrotic ring spot virus infecting Rosa hybrida L. in Egypt |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات بيولوجية وسيرولوجية وجزيئية على فيروس التبقع الحلقى النيكروزى الذى يصيب الورد فى مصر. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.125-138. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Prunus necrotic ring spot virus (PNRSV) was isolated for the first time in Egypt from naturally infected rose plants collected from the experimental farm of the Faculty of Agriculture, Cairo University). Observed symptoms circumvented necrotic ring spots on leaves, bud failure, and color breaking of petals. The virus was transmitted mechanically. The purified virus had Amax and A min at 260 and 240 nm respectively. The 260/280 ratio was 1.56. Yield of purified virus from infected Gompherina globosa was 0.182 mg/g tissue. Electron micrograph of the purified virus showed spherical (23-nm) as well as bacilliform virus particles (42x23 nm). The induced antiserum from the purified virus was successfully used to detect PNRSV in rose plants in several locations in Egypt. The full length of the replicase gene of PNRSV was successfully amplified by reverse transcription-polymerase chain reaction (RT-PCR) using different sets of specific primers. A sensitive and specific IC-RT-PCR protocol was used for the detection of PNRSV from rose tissues. Sequence analysis of PNRSV/rep gene of the rose isolate indicated 60 % similarity to that of PNRSV-AF278534 and NC-004362. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Roses |
| General subdivision | Diseases and pests. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Virus diseases. |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Prunus necrotic ringspot virus. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ibrahim, Ibrahim A. M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdelkader, Hayam S. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mokbel, Samah A. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Shazly, Manal A. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Arab Journal of Biotechnology. |
| Related parts | 2008.v.11(1) |
| International Standard Serial Number | 1110-6875 |
| Control subfield | nnas |
| Record control number | u189256 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2008/AJB/1108/1/125.pdf">http://nile.enal.sci.eg/EALE/2008/AJB/1108/1/125.pdf</a> |
| Public note | Full Text Article |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.