Restriction fragment length polymorphisms reveal a new molecular group of infectious bursal disease virus in Saudi Arabia (Record no. 44462)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02191cab a2200229Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u189789 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110223s2006 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART EPSJ V26 NoI 6 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Alkhalaf, A. N. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Egyptian poultry science journal, 2006 v. 26 (I) |
| Medium | [electronic resouce]. |
| 245 10 - TITLE STATEMENT | |
| Title | Restriction fragment length polymorphisms reveal a new molecular group of infectious bursal disease virus in Saudi Arabia |
| Medium | [electronic resouce]. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.87-92. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Infectious bursal disease virus (IBDV) causes immunosuppression in chickens and high condemnations in slaughter plants. A total of 87 samples were obtained from fifteen commercial chicken flocks and nine backyard chicken flocks that have different ages and vaccination programs. The reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used to detect the viruses and Restriction Fragment Length Polymorphisms (RFLP) was used to compare the RT-PCR products among the viruses that were detected. The restriction enzymes that were used in the RFLP are BstNI and MboI. All backyard flock samples were negative to IBDV. Four samples from commercial flocks were positive for IBDV using the RT-PCR and the RFLP was performed on the RT-PCR product of these samples. Two samples had RFLP bands that were different from any other known RFLP patterns and the other samples had RFLP bands that matched classical vaccine strain patterns. In conclusion, a new molecular group of IBDV may be present in Saudi Arabia that is different from any existing molecular group. Abbreviations: IBDV = Infectious bursal disease virus; RT-PCR = reverse transcriptase-polymerase chain reaction; RFLP = Fragment Length Polymorphisms, DMSO = dimethyl sulfoxide solution, hv = hyper-variable region, AC-ELISA = Antigen capture enzyme linked immunosorbent assay |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Poultry |
| General subdivision | Diseases |
| Geographic subdivision | Saudi Arabia. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Poultry |
| General subdivision | Virus diseases. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Egyptian poultry science journal. |
| Related parts | 2006.v.26(I) |
| International Standard Serial Number | 1110-5623 |
| Control subfield | nnas |
| Record control number | u189066 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2006/EPSJ/2606/I/87.pdf">http://nile.enal.sci.eg/EALE/2006/EPSJ/2606/I/87.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.