Molecular cloning and expression of ESAT-6 antigen of Mycobacterium bovis for differential diagnosis of bovine tuberculosis (Record no. 44760)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01902cab a2200337Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u190159 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110309s2007 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART ZVJ V35 No1 12 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Soliman, Y. A. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Zagazig veterinary journal, 2007 v. 35 (1) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular cloning and expression of ESAT-6 antigen of Mycobacterium bovis for differential diagnosis of bovine tuberculosis |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | كلونة وتعبير الأنتيجين ESAT-6 من ميكروب السل البقرى للتشخيص التفاضلى للسل البقرى. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P. 122-134. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In a search for developing new skin test reagents, the possibility of using ESAT-6 protein antigen as a candidate antigen for the diagnosis of bovine tuberculosis was investigated. Genomic DNA of M. bovis was extracted, purified and the esat-6 gene was amplified by PCR. The gene was then ligated to an expression vector, PQE. After transformation of an E. coli TOPO host strain with the PQE plasmid, the expression was induced using 10 mM of IPTG. A band (18 KDa) was seen in the SDS-PAGE analysis. The His-tagged r ESAT-6 antigen was then purified by metal affinity chromatography using Ni-NTA agarose. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tuberculosis in cattle. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Mycobacterium bovis. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Molecular cloning. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tuberculosis |
| General subdivision | Microbiology. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Mycobacterium bovis. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Enzyme-linked immunosorbent assay. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tuberculosis |
| General subdivision | Diagnosis. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Antigen-antibody reactions. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Zagazig Veterinary Journal. |
| Related parts | 2007.v.35(1) |
| International Standard Serial Number | 1110-1458 |
| Control subfield | nnas |
| Record control number | u189860 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2007/ZVJ/3507/1/122.pdf">http://nile.enal.sci.eg/EALE/2007/ZVJ/3507/1/122.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.