Virological and molecular studies on rotavirus (Record no. 45268)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02422cab a2200313Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u190730 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110328s2009 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART ZVJ V37 No4 7 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ali, Ahmed Abdel Samie H. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Zagazig veterinary journal, 2009 v. 37 (4) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Virological and molecular studies on rotavirus |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات فيرولوجية وجزيئية على فيروس الروتا. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.70-79. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Bovine Rotavirus (BRV) is a major cause of acute gastroenteritis in neonatal calf diarrhea throughout the world causing economic losses to beef and dairy industries. Neomycin B, an aminoglycoside antibiotic has been shown to inhibit human immunodeficiency virus and Herpes simplex virus replication. The current study was carried out to assess the effect of neomycin B on rotavirus replication in Rhesus monkey kidney fetal cells (MA-104) by immunofluorescence (IF), plaque assay and now cytometry. Neomycin interfered with the development of BRV cytopathic effect (CPE). Neomycin concentrations at 5, 7.5 and 10 mM were able to reduce the intracytoplasmic fluorescence induced by BRV byI6.67%. 41,67% and 83.33% respectively. There was marked decrease in the yield of viral RNA. Neomycin concentrations of 5. 7.5 and 10mM were observed to be able to reduce the percentage of cell infectivity with BRV by 16.21 %. 56.72% and 73.94% respectively. The kinetics of BRV growth was carried out by plaque assay. At the concentrations 7.5 and 10mM neomycin. there was marked decrease in virus titer in supernatant at time interval 48 and 72h post infection, It was concluded that Neomycin B is an inhibitor of BRV replication in-vitro particularly when the drug IS added during the complete infection cycle; pre-incubation of virus and pretreatment of MA-104 with neomycin does not affect replication of BRV. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Rotaviruses. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cattle |
| General subdivision | Diseases |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Neomycin. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Salama, Ali A. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zeidan, Sayed M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mansour, Shimaa M.G. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Zagazig Veterinary Journal. |
| Related parts | 2009.v.37(4) |
| International Standard Serial Number | 1110-1458 |
| Control subfield | nnas |
| Record control number | u189860 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2009/ZVJ/3709/4/70.pdf">http://nile.enal.sci.eg/EALE/2009/ZVJ/3709/4/70.pdf</a> |
| Public note | Full Text Article |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.