Evaluation of rose bengal test, gel immunodiffusion with native hapten, and indirect elisa in diagnosis of brucellosis in infected and vaccinated sheep (Record no. 45489)

MARC details
000 -LEADER
fixed length control field 02633cab a2200349Ia 45 0
001 - CONTROL NUMBER
control field u190975
003 - CONTROL NUMBER IDENTIFIER
control field SIRSI
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 110405s2010 ua ss b eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EAL
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
Language code of summary or abstract ara
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) ART AVMJ V56 NO125 10
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Bassiony, M. M.
9 (RLIN) 2792
240 10 - UNIFORM TITLE
Uniform title Assiut veterinary medical journal, 2010 v. 56 (125)
Medium [electronic resource].
245 10 - TITLE STATEMENT
Title Evaluation of rose bengal test, gel immunodiffusion with native hapten, and indirect elisa in diagnosis of brucellosis in infected and vaccinated sheep
Medium [electronic resource].
246 15 - VARYING FORM OF TITLE
Title proper/short title تقييم اختبار الروزبنجال والأنتشار المناعى فى الجل باستخدام الهابتين المحلى وأختبار الاليزا الغير مباشر فى تشخيص البروسيلا فى الأغنام المصابة و المحصنة بالبروسيلا.
300 ## - PHYSICAL DESCRIPTION
Extent p.132-142.
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes references.
520 ## - SUMMARY, ETC.
Summary, etc. Three serological assays were evaluated for the diagnosis of brucellosis in sheep: the Rose Bengal plate test as a screening test (RBPT), agar gel immunodiffusion with Native Hapten test (AGID-NH) and an indirect enzyme-linked immunosorbent assay (iELISA). For optimal sensitivity, RBPT had to be used with sera: antigen at a 3:1 dilution. With the sera from B. melitensis culture-positive sheep, the sensitivity was 100% for RBPT, and iELISA; while, it was 93.8% for AGID-NH test. On the other hand specificity was 100% when testing the sera from 145 brucella-free sheep. Larger discrepancies among the results of the serological tests were obtained with sera from sheep of areas where brucellosis is endemic. Sheep vaccinated with B. melitensis Rev.1 subcutaneously and conjunctively showed negative result in all tests after twelve and six months respectively. The proportion of sheep giving a positive reaction after vaccination decreased faster in AGID-NH test than in other tests. The using of RBPT or ELISA with combination with AGID-NH is the best choice for diagnosis of infected and vaccinated sheep.
546 ## - LANGUAGE NOTE
Language note Summary in Arabic.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Sheep
General subdivision Diseases
-- Diagnosis.
9 (RLIN) 3146
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Sheep
General subdivision Infections.
9 (RLIN) 3147
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Sheep
General subdivision Vaccination.
9 (RLIN) 3148
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Brucellosis in sheep
General subdivision Diagnosis.
9 (RLIN) 379
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Enzyme-linked immunosorbent assay.
9 (RLIN) 368
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Haptens.
9 (RLIN) 3149
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Abou gazia, K. A.
9 (RLIN) 3150
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Abd al-azeem, M. W.
9 (RLIN) 3151
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Ibrahim, I. G. A.
9 (RLIN) 3152
773 0# - HOST ITEM ENTRY
Title Assiut Veterinary Medical Journal.
Related parts 2010.v.56(125)
International Standard Serial Number 1012-5973
Control subfield nnas
Record control number u181356
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="http://nile.enal.sci.eg/EALE/2010/AVMJ/5610/125/132.pdf">http://nile.enal.sci.eg/EALE/2010/AVMJ/5610/125/132.pdf</a>
Public note Full Text Article.
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Articles
Source of classification or shelving scheme Library of Congress Classification

No items available.


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