Construction of a recombinant baculovirus expressing the major capsid protein (VP6) of bovine rotavirus (Record no. 46076)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02583cab a2200337Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u191639 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110427s2007 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AJB V10 No2 13 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | EL-Sabagh, I. M. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Arab journal of biotechnology, 2007 v. 10 (2) |
| Medium | [electronic resource]: |
| -- | an international journal devoted to the advancement of biotechnology. |
| 245 10 - TITLE STATEMENT | |
| Title | Construction of a recombinant baculovirus expressing the major capsid protein (VP6) of bovine rotavirus |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | بناء فيروس مدمج من جنس الباكيلو للتعبير عن بروتين الغلاف الداخلى لفيروس الروتا البقرى. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.369-384. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The present study reports the expression of VP6, the major inner capsid protein of bovine rotavirus Nebraska calf diarrhea virus (NCDV) strain in a baculovirus expression system. The fulllength DNA copies of RNA segment 6 (coding for VP6 protein) of NCDV were inserted into a baculovirus expression vector. A recombinant baculovirus carrying the VP6 gene was constructed through homologous recombination between the baculovirus recombinant plasmid carrying the VP6 gene and Autographa californica nuclear polyhedrosis virus (AcNPV) under the control of the polyhedrin promotor. Infection of Spodoptera frugiperda (Sf9) cells with the recombinant baculovirus expressing VP6 protein revealed a high-level of expression when tested by immunoflurescence and solid phase ELISA tests using BRV-specific polyclonal antibodies. The VP6 expressed protein was detected in Coomassie blue stained SDS-PAGE and produced a detectable band in Western blot assay. The high degree of reactivity with BRV-specific polyclonal antibodies confirmed that the antigenic determinants of the expressed protein were unaltered. The use of the in vitro expressed VP6 protein in the field diagnosis and vaccine development to control rotavirus infection is of considerable interest. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Rotaviruses. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Baculoviruses |
| General subdivision | Biotechnology. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Baculoviruses |
| General subdivision | Genetics |
| -- | Laboratory manuals. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hussein, H. A. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amer, H. M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | EL-Sanousi, A. A. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Reda, I. M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Shalaby, M. A. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Arab journal of biotechnology. |
| Related parts | 2007.v.10(2) |
| International Standard Serial Number | 1110-6875 |
| Control subfield | nnas |
| Record control number | u189256 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2007/AJB/1007/2/369.pdf">http://nile.enal.sci.eg/EALE/2007/AJB/1007/2/369.pdf</a> |
| Public note | Full Text Article |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.