Biological, biochemical, serological, molecular and tissue cultural studies on an Egyptian isolate of tomato spotted wilt virus infecting chrysanthemum plants (Record no. 46478)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03902cab a2200385Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u192815 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110620s2009 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART EJP V37 No2 6 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | El-Shazly, Manal A. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Egyptian journal of phytopathology, 2009 v. 37 (2) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Biological, biochemical, serological, molecular and tissue cultural studies on an Egyptian isolate of tomato spotted wilt virus infecting chrysanthemum plants |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | دراسات بيولوجية، بيوكيميائية، سيرولوجية، جزيئية وزراعة الأنسجة على عزلة مصرية لفيروس الذبول المنقط فى الطماطم التى تصيب نباتات الأراولا. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P. 79-94. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Tomato spotted wilt virus (TSWV) has been isolated and identified from naturally infected chrysanthemum plants, collected from the experimental farm of the Fac. of Agric., Cairo Univ. by indirectenzyme linked immunosorbent assay (Indirect-ELISA) and dot blotting immunobinding assay (DBIA) using an induced antiserum for TSWV. The symptoms consisted of dark coloured leaf necrosis, necrotic line patterns, necrotic local lesion and tip necrosis on leaves, stunting, wilting and flower distortion. All the tested chrysanthemum cultivars were found to be susceptible when mechanically inoculated under greenhouse conditions. Wide variations of symptoms were found between Shakira, Feling, Grancl, Zambia and Maxx cultivars. Cv. Zambia was found to be more susceptible than any other cultivar tested in the present study, thus it was used for the production of virus-free plants using tissue culture technique. TSWV was purified from infected Gompherina globosa plants. The UV absorption spectrum had Amax at 260nm, Amin at 245nm and A₂₆₀/₂₈₀ ratio of 1.2. Coat protein subunit of TSWV had a value of 29 KDa. The IgG fraction of the prepared antiserum had Amax and Amin~~ at 280 and 242 run, respectively. Immunocapture-reverse transcription-polymerase chain reaction (lC- RT-PCR) was used to amplify 760 bp cDNA fragments from infected chrysanthemum leaves using the primers (SLS90-47) and (JLS90-46) specific to TSWV. The dilution end point of TSWV in infected tissue extracts was 1/1280, 1/2560 and 10-s for indirect ELISA, direct ELISA and IC- RT- PCR, respectively. IC- RTPCR proved to be more sensitive than indirect and direct ELISA. In this study, meristem-tips were excised from infected chrysanthemum plants. Thermotherapy and/or chemotherapy were used to eliminate TSWV. Results demonstrated that application of thermotherapy at 38°C for 28 days followed by 10 to 20 mg/l Virazole in vitro gave a survival rate of 53 and 77%, respectively. The presence of virus in the produced plants was evaluated by ELISA technique. In vitro thermotherapy combined with chemotherapy eliminated TSWV from infected chrysanthemum and reduced the risk of introducing this pathogen through vegetative propagation. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tomatoes |
| General subdivision | Diseases and pests. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tomato spotted wilt virus disease. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chrysanthemums |
| General subdivision | Diseases and pests. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Enzyme-linked immunosorbent assay. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Danish Brotherhood in America. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tomato spotted wilt virus disease |
| General subdivision | Chemotherapy. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tomato spotted wilt virus disease |
| General subdivision | Thermotherapy. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tissue culture. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tomatoes |
| General subdivision | Flowering. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Chrysanthemums |
| General subdivision | Flowering. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dawood, Rehab A. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Soliman, A. M. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Egyptian Journal of Phytopathology. |
| Related parts | 2009.v.37(2) |
| International Standard Serial Number | 1110-0230 |
| Control subfield | nnas |
| Record control number | u192592 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2009/EJP/3709/2/79.pdf">http://nile.enal.sci.eg/EALE/2009/EJP/3709/2/79.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.