Molecular characterization of an Egyptian isolate of tomato mosaic tobamovirus (Record no. 51621)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02103cab a2200325Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u198791 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120315s2004 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AAS V49 NO2 4 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | EI-Mfifi, Sobair L |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Annals of agricultural science, 2004 v. 49 (2) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Molecular characterization of an Egyptian isolate of tomato mosaic tobamovirus |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الخصائص الجزيئية لعزلة مصرية من فيروس موزيك الطماطم. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.467-483 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Tomato plant is recorded to be one of the most economical vegetable crops in Egypt This crop was found to be widely and severely infected with tomato mosaic: tobamoviTus (ToMV). An Egyptian isolate of ToMV was subjected to molecular characterization. The virus was purified from tobacco leaves using differential centrifugation. The electron microscopy of purified vims ptepaIlltion showed the presence of rod-shaped vims-like particles with a model size close to 300 X 17 om. The SOS-polyacrylamide gel electrophoresis (SOS-PAGE) showed a single coat protein band with molecular weight of ahout 17 KDa ToMV coat protein gene (cp) was is0lated and amplified using immunocaptwe reverse transeriptase polymerase chain reaction (IC-RT-PCR) and its size length on agarose gel was found to be 479 bp. The nucleotide sequence of the viml genome was studied, as a genome composed of 6383 nucleotides was sequenced and its organization was addressed. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Tomatoes |
| General subdivision | Diseases and pests |
| -- | Control. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Mosaic viruses |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Virus diseases of plants. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Genomics |
| General subdivision | Research |
| -- | Methodology. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Sadik, A.S. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abdel-Gbaffar, M.R |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | EI-Borollosy, A.M. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Annals of Agricultural Science. |
| Related parts | 2004.v.49(2) |
| International Standard Serial Number | 0570-1783 |
| Control subfield | nnas |
| Record control number | u157971 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2004/AAS/4904/2/467.pdf">http://nile.enal.sci.eg/EALE/2004/AAS/4904/2/467.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
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