Application of in situ hybridization in diagnosis of brucellosis in tissues of naturally infected sheep and goats (Record no. 52098)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02682cab a2200325Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u199398 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 101222s2004 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AVMJ V50 No101 9 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Montasser, A. M. |
| 9 (RLIN) | 1838 |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Assiut veterinary medical journal, 2004 v. 50 (101) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Application of in situ hybridization in diagnosis of brucellosis in tissues of naturally infected sheep and goats |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تطبيق التهجين الموضعي في تشخيص مرض البروسيلا في أنسجة الأغنام و الماعز المصابة طبيعيا بالمرض. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.110-125. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Brucellosis in sheep and goats are very common in Egypt and can be diagnosed by bacteriological and serological techniques. The present study was carried out for the assessment of sensitivity and specificity of in situ hybridization (ISH). One hundred animals (50 sheep and 50 goats) from an area with history of brucella infection were included and examined serologically by Buffered acidified plate test (BAP AT) Rose Bengal plate test (RBPT), Serum agglutination test (SAT), rivanol test and complement fixation test (CFT). Out of them 19 animals were reactors serologically by one or more test (10 from sheep and 9 from goats). From 19 reactors animals, proper samples from spleen and lymph nodes were collected for bacteriological, histopathological examination and ISH techniques. The results obtained from isolation trails, 7 brucella isolets were isolated (4 from sheep and 3 from goats). The isolated straines typed as Brucella melitensis biovar 3. The results obtained by ISH on paraffin embedded section of lymph nodes using a digoxigenin labeled DNA fragment located at gene encoding at 31 KDa Brucella abortus protein, revealed 12 cases out of 19 reactors animals were positive lymph nodes. The sensitivity of ISH techniques was higher than that of microbial culture where as the specificity of ISH was 100% when compared with the conventional microbial culture. Key words: Hybridization, Brucellosis, Sheep, Goats. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Sheep |
| General subdivision | Diseases. |
| 9 (RLIN) | 1839 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Goats |
| General subdivision | Diseases. |
| 9 (RLIN) | 1840 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Hybridization. |
| 9 (RLIN) | 1841 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Brucellosis in goats. |
| 9 (RLIN) | 379 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Brucellosis in sheep. |
| 9 (RLIN) | 379 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Helmy, Nashwa M. |
| 9 (RLIN) | 496 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ahmed, Hanem M. |
| 9 (RLIN) | 1842 |
| 773 0# - HOST ITEM ENTRY | |
| Title | Assiut Veterinary Medical Journal. |
| Related parts | 2004.v.50(101) |
| International Standard Serial Number | 1012-5973 |
| Control subfield | nnas |
| Record control number | u181356 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2004/AVMJ/5004/101/110.pdf">http://nile.enal.sci.eg/EALE/2004/AVMJ/5004/101/110.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.