PCR cloning of polyhydroxybutyrate synthase gene (PhbC) from Aeromonas hydrophila (Record no. 52696)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02317cab a2200337Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u200108 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120510s2004 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AJB V7 No2 1 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Enan, M. R. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Arab journal of biotechnology, 2004 v. 7 (2) |
| Medium | [electronic resource]: |
| -- | an international journal devoted to the advancement of biotechnology. |
| 245 10 - TITLE STATEMENT | |
| Title | PCR cloning of polyhydroxybutyrate synthase gene (PhbC) from Aeromonas hydrophila |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | إستنساخ جين السينسيز المسؤل تخليق مركب البولى هيدروكسى بيوتيرات من بكتيريا الإيرومونس هيدروفيلا بإستخدام التفاعل التسلسلى للبوليميراز. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P. 157-164. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by a high production cost. A Polymerase Chain Reaction (PCR) protocol was developed for the specific detection and isolation of full-length gene coding for polyhydroxybutyrate. PCR strategy using PHB primers resulted in the amplification of DNA fragments with the expected size from all isolated bacteria. PHB synthase gene was cloned directly from Aeromonas hydrophila genome for the first time from these bacteria. The cloned fragment was named phb CAh. This fragment was partially sequenced and open reading frame was found representing the PHB synthase gene. The nucleotide sequence of phbCAh gene exhibits similarity to Caulobacter crescentus phbC (40.3%), Pseudomonas oleovorans phbC (34.3%), and Rhodobacter sphaeroides phbC (40.6 %). |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Aeromonas hydrophila. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plastic scrap. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Genomes. |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Polymerase chain reaction. |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Polyhydroxybutyrate. |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Pseudomonas oleovorans. |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Biodegradable products. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Bashandy, S. A. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Arab Journal of Biotechnology. |
| Related parts | 2004.v.7(2) |
| International Standard Serial Number | 1110-6875 |
| Control subfield | nnas |
| Record control number | u189256 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2004/AJB/704/2/157.pdf">http://nile.enal.sci.eg/EALE/2004/AJB/704/2/157.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.