Production, purification, characterization and immunological studies of E.coli E-U₁₀ penicillin amidase (Record no. 57052)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 01555cab a2200241Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u206868 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110417s2001 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AJVS V17 No1 1 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Temsah, S. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Alexandria journal of veterinary science, 2001 v. 17 (1) |
| Medium | [electronic reource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Production, purification, characterization and immunological studies of E.coli E-U₁₀ penicillin amidase |
| Medium | [electronic reource]. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.1-16. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Penicillin amidase enzyme (PA) was isolated from the culture filtrate of one isolated strain of E. coli E-U₁₀ grown on optimized medium after 24 hours incubation. The enzyme was purified to homogeneity by sequential ammonium sulfate fractionation and ion exchange and gel filtration chromatography. On using Disc-PAGE, the final purification fraction showed only one distinctive band indicating high purity. On using SDS-PAGE the final fraction showed two distinctive units with molecular weight of 22000 and 59000 Dalton. The optimal pH, temperature and time course for activity were 7.5, 37°C and 10 minutes respectively. The enzyme with penicillin G as substrate had a Km of 13 mM and Vmax 3.92 U/ml. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Escherichia coli. |
| 653 ## - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | penicillin amidase. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mabrouk, A. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Alexandria Journal of Veterinary Science. |
| Related parts | 2001.v.17(1) |
| International Standard Serial Number | 1110-2047 |
| Control subfield | nnas |
| Record control number | u191343 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2001/AJVS/1701/1/1.pdf">http://nile.enal.sci.eg/EALE/2001/AJVS/1701/1/1.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
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