Detection of six E.coli O157 virulence genes in water samples using multiplex PCR (Record no. 59917)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02331cab a2200337Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u210715 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 110120s2012 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART EJM V47 11 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | El-Leithy, M. A. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Egyptian journal of microbiology, 2012 v.47 |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Detection of six E.coli O157 virulence genes in water samples using multiplex PCR |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الكشف عن ستة جينات شديدة الاصابة فى الايشريشيا كولاى O157 فى عينات المياه باستخدام تفاعل البلمره المتعدد. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.171-188. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Escherichia coli O157 strains have emerged as important human enteric pathogens. E.coli O157 strains may be transmitted in a variety of ways, including drinking water, recreational water and wastewater. One hundred and seventy five water samples were collected from different water sources from June 2010 to July 2011 and examined for classical bacterial indicators (total bacterial counts at 37°C and 22°C, total coliforms, fecal coliforms and fecal streptococci) and E.coli O157. Total coliforms (TC), fecal coliforms (FC) and fecal streptococci (FS) among the collected water samples were 83, 76 and 76 out of 175 (MPN- index/100 ml) with incidence ratio of 47%, 43% and 43%, respectively. Escherichia coli O157 was detected in water samples using HiCrome EC O157:H7 agar and multiplex PCR targeting six virulence genes (stxl (Shiga toxin 1 gene), stx2 (Shiga toxin 2 gene), eae (intimin gene), hlyA (hemolysin gene), rfbE (O157 antigen gene), and fliC (flagellar antigen gene)}. Keywords: E.coli O157, Virulence gene, Multiplex PCR, Water |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Escherichia coli. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Polymerase chain reaction. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Virulence (Microbiology) |
| General subdivision | Genetic aspects. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Water |
| General subdivision | Pollution. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Shatoury, Einas H. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Senousy, W. M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abou-Zeid, M. A. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Taweel, Gamila E. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Egyptian Journal of Microbiology. |
| Related parts | 2012.v.47 |
| International Standard Serial Number | 0022-2704 |
| Control subfield | nnas |
| Record control number | u184012 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2012/EJM/4712/0/171.pdf">http://nile.enal.sci.eg/EALE/2012/EJM/4712/0/171.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.