Detection of Staphylococcus Aureus Enterotoxins in milk, milk products as well as milk handlers in Sharkia governorate (Record no. 60852)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03817cab a2200265Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u211915 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120313s2012 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AASM V50 No4 2 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Abd El-Wahed, Elsayed M |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Annals of agricultural science, Moshtohor, 2012 v.50 (4) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Detection of Staphylococcus Aureus Enterotoxins in milk, milk products as well as milk handlers in Sharkia governorate |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | الكشف عن السموم المعوية لميكروب ستافيلوكوكس أوريس في الألبان ومنتجاتها والقائمين بتداولها في محافظة الشرقية. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.417-426. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Staphylococus aureus may contain one or more genes that encode staphylococcal enterotoxins (SE) that cause food poisoning. The previously known toxins were the five major classical types; however, with the extensive analysis of the Staph. aureus genome, new genes encoding enterotoxia-like superantigens have been identified. Milk and dairy products are frequently contaminated with enterotoxigenic Staph. aureus, which is often involved in staphylococcal food poisoning; these contaminations are either from animal or human sources. This work aimed to detect types of enterotoxins produced by Staph, aureus isolated from milk, kariesh cheese and ice-cream samples and from nasal swabs got from food handlers, the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and PCR was used. In this work, 450 samples of Milk, ice-cream, kariesh cheese and nasal swabs from food handlers were examined for the presence of Coagulase positive Staph. aureus, using Mannitol salt agar, Baird-Parker agar, tube coagulase test, and latex agglutination test for protein A and capsular polysaccharides. Confirmed Staph. aureus isolates were examined for the production of SEs using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and the type of SE genes by polymerase chain reaction (PCR). The Coagulase positive Staph. aureus isolates were detected in 84% of Staph colonized raw milk, 87.5% of Staph colonized ice-cream and 92.8% of Staph colonized kariesh cheese samples and 50% of Staph colonized nasal swabs, with 62.5% of total staph colonization which exceeds the Egyptian Standards. Collectively, 49.5% of coagulase positive Staph. aureus isolates were enterotoxigenic and the highest percentages were detected in raw milk taken directly from animals (68.7%) and kariesh cheese from street distributors (65.7%). In all samples, the major classical enterotoxin genotype was SEA which was detected in 33 isolates of toxigenic isolates. SEC was detected in 17 isolates and SED in II isolates. SEB could not be detected. For the newly described genes, SEG was detected in II isolates and SEH in 8 isolates. Mixed forms were found in 25 isolated of toxigenic isolates and four strains can’ied undescribed genes. Therefore we concluded that: Raw milk and some dairy products in the markets in Sharkia Governorate, Egypt are contaminated with enterotoxigenic Staph. aureus. The most common type in both milk and dairy products as well as in nasal swabs was SEA which is known to be less common among strains from animal origin than from human. Nasal carriage in human food handlers is considered a primary source of contamination of milk and dairy products. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Dairy microbiology. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Staphylococcus aureus |
| 773 0# - HOST ITEM ENTRY | |
| Title | Annals of Agricultural Science, Moshtohor. |
| Related parts | 2012.v.50(4) |
| International Standard Serial Number | 1110-0419 |
| Control subfield | nnas |
| Record control number | u183870 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2012/AASM/5012/4/417.pdf">http://nile.enal.sci.eg/EALE/2012/AASM/5012/4/417.pdf</a> |
| Public note | Full Text Article |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.