Multiplex PCR and latex agglutination for identification and characterization of toxigenic clostridium difficult from raw milk (Record no. 61124)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02289cab a2200361Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u212350 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160811s2014 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AVMJ V60 No143 7 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | El-Leboudy, Ahlam A. |
| 9 (RLIN) | 282 |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Assiut veterinary medical journal, 2014 v. 60 (143) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Multiplex PCR and latex agglutination for identification and characterization of toxigenic clostridium difficult from raw milk |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | تفاعل البلمرة المتسلسل المتعدد واختبار التجلط للتعرف على وتمييز الكلوستريديام ديفيسيل فى اللبن الخام. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.49-53. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Community-acquired C.Diff infections have recently been increasing in incidence and severity. Several studies have isolated C. Diff spores from livestock and food suggesting that food may play a role in transmission. The overall goals of this research were to: 1) determine prevalence of C. Diff from raw milk using selective (cycloserine-cefoxitin fructose agar [CCFA]; 2) determine toxigenic profiles of C.Diff. isolated using PCR targeting tpi and tcdB genes. A total of 50 random samples of raw marketable milk were collected from different localities in Assuit city, Egypt. The obtained results indicated that about 11 (22%) out of 50 examined raw milk samples were positive for C.Diff using conventional method. While, 2 strains of C.Diff. (18.2%) out of the positive 11 strains could be detected using the latex agglutination test. Keywords: Clostridium Difficile, Raw milk, latex agglutination, toxigenic, PCR. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Raw milk. |
| 9 (RLIN) | 283 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Clostridium diseases. |
| 9 (RLIN) | 284 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Milk |
| General subdivision | Microbiology. |
| 9 (RLIN) | 285 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Polymerase chain reaction. |
| 9 (RLIN) | 250 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Agglutination tests. |
| 9 (RLIN) | 286 |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Clostridium difficile |
| 9 (RLIN) | 287 |
| 653 ## - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | PCR |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Saad, Nagah M. |
| 9 (RLIN) | 187 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Amer, A. |
| 9 (RLIN) | 288 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Elmoazen, Noha S. |
| 9 (RLIN) | 289 |
| 773 0# - HOST ITEM ENTRY | |
| Title | Assiut Veterinary Medical Journal. |
| Related parts | 2014.v.60(143) |
| International Standard Serial Number | 1012-5973 |
| Control subfield | nnas |
| Record control number | u181356 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2014/AVMJ/6014/143/49.pdf">http://nile.enal.sci.eg/EALE/2014/AVMJ/6014/143/49.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.