ITS2 region-based molecular identification of fungal pathogens in equine corneal ulceration (Record no. 61126)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 03029cab a2200385Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u212353 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160811s2014 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AVMJ V60 No143 9 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Hamed, Maha I. |
| 9 (RLIN) | 246 |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Assiut veterinary medical journal, 2014 v. 60 (143) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | ITS2 region-based molecular identification of fungal pathogens in equine corneal ulceration |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التححديد الجزيئى القائم على المنطقة ITS2 للفطريات الممرضة المسببة لتقرح القرنية فى الخيول. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p.61-69. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This study aimed to assess the utility of polymerase chain reaction (PCR) and DNA sequencing in diagnosing fungal ulcerative keratitis in horses and compare their sensitivity with conventional microbiologic techniques used in laboratories. Conjunctival swabs from 12 horses with corneal ulcerations admitted to the Veterinary Teaching Hospital, College of Veterinary Medicine, Purdue University were collected for examinations. 14 conjunctival swabs were analyzed by the conventional culture method and by generic PCR using universal fungal primers to amplify the internal transcribed spacer 2 (ITS2) genetic region followed by DNA sequencing. The conventional culture method revealed that two samples exhibited fungal growth that identified as Aspergillus sp. and Penicillium sp. while three conjunctival samples contained bacterial growth which was identified as Staphylococcus intermedius, Staphylococcus epidermidis and Streptococcus zooepidemicus. Interestingly, PCR followed by DNA sequencing of the biological specimens on the swabs identified fungal pathogens in 9/14 samples and Ascomycete species in 3/14 samples. In 2/14 samples, no fungal pathogen was identified using PCR. Fungal pathogens detected included Aspergillus sp. in six eyes, Penicillium sp. in one eye, Fusarium sp. in one eye and Cladosporium sp. in one eye. Our findings demonstrate a limitation of the conventional culture method as a diagnostic tool as a fungal pathogen was detected in only two samples by this method as compared to nine samples using PCR and DNA sequencing. Key words: ITS2 region, fungal pathogens, equine, corneal ulcers. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Cornea |
| General subdivision | Ulcers. |
| 9 (RLIN) | 247 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Corneal diseases |
| 9 (RLIN) | 248 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Horses |
| General subdivision | Diseases |
| -- | Treatment. |
| 9 (RLIN) | 249 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Pathogenic fungi |
| 9 (RLIN) | 213 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Polymerase chain reaction. |
| 9 (RLIN) | 250 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | DNA |
| General subdivision | Therapeutic use. |
| 9 (RLIN) | 251 |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | internal transcribed spacersg |
| 9 (RLIN) | 252 |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | opacity |
| 9 (RLIN) | 253 |
| 653 ## - INDEX TERM--UNCONTROLLED | |
| Uncontrolled term | corneal ulceration |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mohammad, Haroon. |
| 9 (RLIN) | 254 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Townsend, Wendy. |
| 9 (RLIN) | 255 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Seleem, Mohamed N. |
| 9 (RLIN) | 256 |
| 773 0# - HOST ITEM ENTRY | |
| Title | Assiut Veterinary Medical Journal. |
| Related parts | 2014.v.60(143) |
| International Standard Serial Number | 1012-5973 |
| Control subfield | nnas |
| Record control number | u181356 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2014/AVMJ/6014/143/61.pdf">http://nile.enal.sci.eg/EALE/2014/AVMJ/6014/143/61.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
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