Purification, serology and molecular detection of Egyptian isolates of banana bunchy top babuvirus and faba bean necrotic yellows nanovirus (Record no. 62224)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02331cab a2200361Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u199498 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120417s2004 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AJB V7 No1 14 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Abd El-Salam, Aly M. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Arab journal of biotechnology, 2004 v. 7 (1) |
| Medium | [electronic resource]: |
| -- | an international journal devoted to the advancement of biotechnology. |
| 245 10 - TITLE STATEMENT | |
| Title | Purification, serology and molecular detection of Egyptian isolates of banana bunchy top babuvirus and faba bean necrotic yellows nanovirus |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | التنقية والكشف بالطرق السيرولوجية والجزيئية على فيروسى تورد القمة فى الموز واصفرار وموت الفول البلدى. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P. 141-155. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Two isolates of faba bean necrotic yellows nanovints (FBNYV) and banana bunchy top babuvints (BBTV) were purified. Cellulase added to the extraction buffers (EB) enhanced extraction of both vintses. An acidic pH was used for FBNYV extraction. Several trials for purification of BBTV indicated that alkaline condition surpassed acidic condition in virus extraction. Polyethylene glycol and NaCI were used to concentrate both viruses. Electroeilition coupled with freezing and thawing were employed in further purification steps. Purified FBNYV yielded 677 ~g/ g tissue and with A 260/280 ratio of 1.43. Purified BBTV yielded 12.9 ~g /g tissue and with A 260/280 ratio of 1.3. Both viruses had a single-capsid protein of 20 kDa. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Banana bunchy top disease. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Bananas |
| General subdivision | Diseases and pests |
| -- | Biological control. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Enzyme-linked immunosorbent assay. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Polymerase chain reaction |
| General subdivision | Diagnostic use. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Serology |
| General subdivision | Mathematical models. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Fava bean |
| General subdivision | Diseases and pests |
| -- | Biological control. |
| 650 #3 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Faba bean necrotic yellows virus. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abd El-kader, Hayam S. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Saghir, Salama M. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Hussien, Mona H. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Arab Journal of Biotechnology. |
| Related parts | 2004.v.7(1) |
| International Standard Serial Number | 1110-6875 |
| Control subfield | nnas |
| Record control number | u189256 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2004/AJB/704/1/141.pdf">http://nile.enal.sci.eg/EALE/2004/AJB/704/1/141.pdf</a> |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
No items available.