Polymorphism of IGF-1 and its receptor (IGF-1R) genes and their association with fertility problems of Egyptian buffaloes (Record no. 64024)
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| 000 -LEADER | |
|---|---|
| fixed length control field | nab a22 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | ENAL |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 040319s2018 ua n ss b a eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| Transcribing agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AJVS V59 No1 14 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ramadan, Sherif I. |
| 9 (RLIN) | 24388 |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Alexandria journal of veterinary science, 2018 v. 59 (1) |
| Medium | [electronic reource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Polymorphism of IGF-1 and its receptor (IGF-1R) genes and their association with fertility problems of Egyptian buffaloes |
| Medium | [electronic reource]. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p. 119-124. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The objectives of our study were to detect genetic polymorphisms in insulin-like growth factor-1 (IGF-1) and insulin-like growth factor1- receptor <br/>(IGF-1R) genes by using SCCP and direct sequence methods and to investigate their possible associations with fertility problems in Egyptian buffaloes. <br/>A total number of 100 buffalo heifers at Mahallet Mousa farm in Kafr El-sheikh province, Egypt were investigated. Animals were assigned into two main <br/>groups: normal fertile (50 animals) and infertile due to anestrous (50 animals). Blood samples were collected and the genomic DNA was extracted then PCR <br/>were performed with annealing temperatures at 58oC and 55oC for IGF1 and IGF1-R respectively. SSCP was performed for all amplified samples and loaded <br/>into a non-denaturing 12% polyacrylamide gel. Four amplicon from both of IGF-1 and IGF-1R genes for the two investigated groups were sequenced using <br/>the same primers. 265-bp from 5-untranslated region (5-UTR) of IGF-1 gene and 311-bp from IGF-1R (part of intron 12 and part of exon 13) were amplified. <br/>In all investigated samples there was one SSCP pattern for IGF-1 and for IGF-1R genes in anestrus and normal fertile buffaloes’ groups Moreover there <br/>were no polymorphisms neither in IGF-1 nor IGF-1R after the sequencing of four samples from each buffaloes’ group for both of IGF-1 and IGF-1R genes. <br/>It seems that IGF-1 and IGF-1R are highly conserved in the investigated buffalo’s population.<br/> |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Genetic polymorphisms. |
| 9 (RLIN) | 24389 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Insulin-like growth factor-binding proteins. |
| 9 (RLIN) | 24390 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Buffaloes |
| General subdivision | Growth |
| 9 (RLIN) | 24391 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Buffaloes |
| General subdivision | Genetics |
| 9 (RLIN) | 24392 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Abo-Gamil, Zienab H. |
| 9 (RLIN) | 514 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Qaliouby, Hadeel S. |
| 9 (RLIN) | 24393 |
| 773 0# - HOST ITEM ENTRY | |
| Title | Alexandria Journal of Veterinary Science. |
| Related parts | 2018.v.59(1) |
| International Standard Serial Number | 1110-2047 |
| Control subfield | nnas |
| Record control number | u191343 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://nile.enal.sci.eg/EALE/2018/AJVS/5918/1/119.pdf |
| Public note | Full Text Article. |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Articles | AR |
| Source of classification or shelving scheme | alc |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Date acquired | Total Checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Alphabetical Classification | Main | Main | 03/04/2019 | ART AJVS V59 NO1 14 | 03/04/2019 | 03/04/2019 | Articles |