[electronic resource] (Record no. 65840)
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| 000 -LEADER | |
|---|---|
| fixed length control field | nab a22 7a 4500 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200818s2019 ua |||pss|||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| Transcribing agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART NRMJ V3 NO6 10 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Ali, Safaa M. |
| 9 (RLIN) | 31782 |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Novel research in microbiology journal, 2019 v.3 (6) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | New source of cellulase production using a metagenomic technique |
| Medium | [electronic resource] |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | p. 590-597. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes bibliographic reference |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The cellulase enzymes with high effectiveness under conditions agreeable to the industrial processes<br/>necessities are one of the keys for the successful development of chemical and drug synthesis. The soil<br/>metagenome is an affluent source for the discovery of new natural products. The objective of the current study<br/>was to identify the isolated functional gene(s) of the cellulase enzyme by using metagenomics. The plan of work<br/>composed of collection of different soil samples, isolation of total DNA, fragmentation, cloning, and expression<br/>of the isolated gene(s) in the suitable host microorganism. The total genomic DNA was extracted using a kit<br/>(QIAGEN), and then digested by different restriction enzymes BamHI. The digested fragments ranging from<br/>~300-5000 bp were ligated, cloned into pUC19 vector, and then transformed into Escherichia coli DH5?. The<br/>resulting clones were screened as cellulase producers using a qualitative method. The positive clones which<br/>showed hydrolysis on the plate were screened once more in Luria-Bertani (LB) medium. The plasmids were<br/>isolated and then tested using universal primer (M13), to detect the fragment size and sequence for the<br/>Polymerase Chain Reaction (PCR) products. This study establishes an effortless and professional method for<br/>cloning of recent cellulase genes through ecological metagenomes. In the outlook, the metagenomic guide<br/>approachs may be functional to the elevated selection of novel cellulase from the environment.<br/> |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | cellulase production |
| 9 (RLIN) | 31783 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Soliman, Nadia A. |
| 9 (RLIN) | 31784 |
| 773 0# - HOST ITEM ENTRY | |
| Title | Novel Research in Microbiology Journal |
| Related parts | 2019.v.3(6) |
| Control subfield | 2537-0294 |
| Record control number | 65799 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | http://nile.enal.sci.eg/EALE/2019/NRMJ/319/6/590.pdf |
| Public note | Full Text Article |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | alc |
| Articles | AR |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Date acquired | Total Checkouts | Full call number | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Alphabetical Classification | Main | Main | 08/18/2020 | ART NRMJ V3 NO6 10 | 08/18/2020 | 08/18/2020 | Articles |