Effect of level and time of l-arginine addition to semen extender on the freezability and fertilizing potentials of buffalo spermatozoa | [electronic resource] (Record no. 66491)

MARC details
000 -LEADER
fixed length control field 03200nab a2200325 4500
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210119s2020 ua ||||ss|||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency EAL
Transcribing agency EAL
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
Language code of summary or abstract ara
090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN)
Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) ART AVMJ V66 No166 3
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Badr, M. R.
9 (RLIN) 32935
240 10 - UNIFORM TITLE
Uniform title Assiut veterinary medical journal, 2020 v. 66 (166)
Medium [electronic resource].
245 10 - TITLE STATEMENT
Title Effect of level and time of l-arginine addition to semen extender on the freezability and fertilizing potentials of buffalo spermatozoa<br/>
Medium [electronic resource]
246 15 - VARYING FORM OF TITLE
Title proper/short title تأثير مستوى و زمن إضافة الارجنين لمخففات السائل المنوي على قابلية التجميد و إخصاب الحيوانات المنوية للجاموس
300 ## - PHYSICAL DESCRIPTION
Extent 19-30 p.
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographic reference.
520 ## - SUMMARY, ETC.
Summary, etc. The objective of this study was to investigate the profit of L-arginine addition to the freezing extender on freezability, lipid peroxidation, and fertilizing potentials of frozen-thawed buffalo spermatozoa. Semen was collected with artificial vagina from five adult fertile bulls and diluted with Tris-base extender containing different L-arginine concentrations (0, 0.5, 1, 5 and 10 mM) added during the dilution or after equilibration for 2 h. Diluted semen was cooled to 4oC throughout one hour, equilibrated for 2 h and then frozen in 0.25 ml straws, prior to be stored in liquid nitrogen. Cryopreserved spermatozoa were assessed for post-thawing sperm motility, viability index, acrosomal integrity, lipid peroxidation and fertility rate. The current results clearly indicated that adding 0.5 mM L- arginine to the freezing extender after the<br/>equilibration period significantly (P< 0.05) improved post-thawing motility, viability index and maintain acrosomal integrity (61.66±10.14%, 130.83±9.62 and 19.33±4.82%, respectively) compared with the control semen (33.33±4.40%, 88.33±11.03 and 33.67±3.28%, respectively). Moreover, addition of 0.5 mM L- arginine to the freezing extender after equilibration period significantly diminished (P< 0.05) lipid peroxidation (11.33±3.28 nmol/109) compared with the control (26.67±3.18 nmol/109). All of these<br/>previously enhanced semen characteristics were reflected positively on its fertilizing potentials. The present results revealed that addition of 0.5 mM L- arginine to the freezing extender after 2 h equilibration (short time exposure) might improve semen quality, preserve<br/>the fertilizing potentials and reduce cryodamage of the buffalo spermatozoa.<br/>Keywords : L-arginine, buffalo, spermatozoa, cryopreservation, lipid peroxidation.
546 ## - LANGUAGE NOTE
Language note Summary in Arabic
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Cattle
General subdivision Spermatozoa
9 (RLIN) 1665
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Lipid Peroxidation
9 (RLIN) 19394
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Cryopreservation of organs, tissues, etc.
9 (RLIN) 32944
650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element Cryopreservation
653 #0 - NAL Term
Uncontrolled term L-Arginine
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Abdel-Khalek, Abdel-Khalek E.
9 (RLIN) 17921
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sakr, A. M.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Hegazy, M. M.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Rawash, Z. M.
773 0# - HOST ITEM ENTRY
Title Assiut Veterinary Medical Journal.
Related parts 2020.v.66(166)
International Standard Serial Number u181356
Record control number 1012-5973
Control subfield nnas
856 40 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier http://nile.enal.sci.eg/EALE/2020/AVMJ/6620/166/19.pdf
Public note Full Text Article
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme alc
Articles AR
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Date acquired Total Checkouts Full call number Date last seen Price effective from Koha item type
    Alphabetical Classification     Main Main 01/19/2021   ART AVMJ V66 No166 3 01/19/2021 01/19/2021 Articles

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