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Molecular characterization and identification of five Egyptian pomegranate cultivars. [electronic resource].

By:

EL-Sayed, N.E

Contributor(s):

Shaaban,Mokhtar M

Language: English Summary language: Arabic Description: p.31-41Other title:

تحديد الخصائص الجزيئية والتميز بين خمسة أصناف مصرية من الرمان [Added title page title]

Uniform titles:

Assiut journal of agricultural sciences, 2008 v. 39 (4) [electronic resource].

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In: Assiut journal of agricultural sciences 2008 v. 39 (4)Summary: Random amplified polymorphic DNA (RAPD) analysis was performed on five local cultivars of pomegranate (Punica granatum), which vary in fruit splitting resistance, grown in the farm of Faculty of Agriculture, Assiut University. The cultivars investigated were Manfalouty,Nab-Elgamal, Wardy, Araby and Higazy. Five selected primers, namely OPC7, OPA-3, OPE 7, OPI-2and OPO12,produced 52 DNA fragments in total, and among which 29 fragment(55.76%) were reproducible polymorphic amplified products while, 23 DNA fragments (44.23%) were conserved among the five cultivars. The degree of polymorphism detected made possible the identification of each cultivar by combining the RAPD banding patterns of the five primers. The amplification products obtained With the five primers used to identify cultivar- specific markers and to estimate the genetic relationships among pomegranate genotypes.

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Random amplified polymorphic DNA (RAPD) analysis was performed on five local cultivars of pomegranate (Punica granatum), which vary in fruit splitting resistance, grown in the farm of Faculty of Agriculture, Assiut University. The cultivars investigated were Manfalouty,Nab-Elgamal, Wardy, Araby and Higazy. Five selected primers, namely OPC7, OPA-3, OPE 7, OPI-2and OPO12,produced 52 DNA fragments in total, and among which 29 fragment(55.76%) were reproducible polymorphic amplified products while, 23 DNA fragments (44.23%) were conserved among the five cultivars. The degree of polymorphism detected made possible the identification of each cultivar by combining the RAPD banding patterns of the five primers. The amplification products obtained With the five primers used to identify cultivar- specific markers and to estimate the genetic relationships among pomegranate genotypes.

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