Gene transfer among different species of staphylococcus isolated from human [electronic resource].

Includes reference.

Twenty one Staphylococcus isolates were isolated from Zagazig University Hospital from patients belong different locations in Sharkia. All these isolates were isolated from different parts of human body and identified genetically and microbiologically. The isolates were obtained and characterized in the Dept. of Medical Microbiology Zagazig University. Resistance to antibiotics (streptomycin, chloramphenicol, penicillin and rifampicin) was examined for all of isolate~. The highest conjugation frequencies were 2.14xlO-8 between S. aureus 5 and Staphylococcus spp. 12. This study shows that transferring of genens among different species of Staphylococcus bacteria isolated from different parts of human body can occur. Conjugation frequencies have been enhanced when plates were incubated at 25"c for 48 hour. Addition of calcim chloride increased the frequency about 12.70 fold increases than control. Numbers of donor and recipient with a ratio of 2 : 1 ml/ml resulted in maximum number of transconjugants.

Summary in Arabic.

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