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Genetic identification of some Egyptian Vicia faba L. cultivars and their relation to orobanche crenata l. tolerance [electronic resource].

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Fahmy, Eman M

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Language: English Summary language: Arabic Description: p.123-140Other title:

التعرف الوراثي لبعض أصناف الفول البلدي المصري وعلاقتهم بتحمل الهالوك [Added title page title]

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Journal of biological chemistry and environmental sciences, 2008 v. 3 (1) Part I [electronic resource].

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In: Journal of Biological Chemistry and Environmental Sciences 2008.v.3(1)ISummary: Ten Egyptian faba bean cultivars were assessed for Orobanche tolerance in a field trial during the winter season 2002-2003. Two traits related to Orobanche infection were estimated i.e., number of spikes/plant and weight of Orobanche stems/plant. Statistical analysis revealed differences among the studied cultivars in relation to Orobanche tolerance/susceptibility. The results confirmed that Giza 843 and Misrl were the most tolerant cultivars, while Giza 643, Giza716 and Giza717 were the most susceptible ones. SDS-PAGE and RAPD analysis were used to study the genetic relationships among the ten faba bean cultivars and to detect some molecular genetic markers for Orobanche tolerance /susceptibility. Both SDSPAGE and RAPD analysis revealed distinctive bands that successfully determined tolerant from susceptible bean cultivars. Data analyses for both SDS-PAGE and RAPD-PCR were used to construct a dendrogram showing the genetic relationships among the faba bean cultivars and fitted well with their pedigrees.

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Ten Egyptian faba bean cultivars were assessed for Orobanche tolerance in a field trial during the winter season 2002-2003. Two traits related to Orobanche infection were estimated i.e., number of spikes/plant and weight of Orobanche stems/plant. Statistical analysis revealed differences among the studied cultivars in relation to Orobanche tolerance/susceptibility. The results confirmed that Giza 843 and Misrl were the most tolerant cultivars, while Giza 643, Giza716 and Giza717 were the most susceptible ones. SDS-PAGE and RAPD analysis were used to study the genetic relationships among the ten faba bean cultivars and to detect some molecular genetic markers for Orobanche tolerance /susceptibility. Both SDSPAGE and RAPD analysis revealed distinctive bands that successfully determined tolerant from susceptible bean cultivars. Data analyses for both SDS-PAGE and RAPD-PCR were used to construct a dendrogram showing the genetic relationships among the faba bean cultivars and fitted well with their pedigrees.

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