The role of basal culture media, ammonium citrate and sucrose concentrations in in vitro shoot proliferation of date palm EV. Om El-Ferakh [electronic resource].

Includes reference.

Procedures were developed for micro propagation of date palm (Phoenix dactylifera L.) cv. Om El-Ferakh through direct organogenesis. Results indicated that Beauchesne medium was superior in terms of number of proliferated shoots/explant compared to MS medium either at full strength or half strength macroelements. Beauchesne medium supplemented with 117 roM sucrose exhibited the highest number of proliferated shoot/explant compared to sucrose at 88, 146 or 175 mM. MS mediuf11 at full strength supplemented with 175 mM sucrose gave the lowest a"Verage number of shoots/ explant. The highest average shoot length (em) was obtained with Beauchesne medium and 1/2 strength macroelcrnel1ts MS supplemented with 88.0 mM sucrose Moreover, results also revealed that addition of ammonium citrate at 1.0 mM to Beauchesne medium markedly improved the shoot proliferation and increased average number and length of the obtained shoots. The highest rooting percentage was obtained with half strength MS mediam supplemented with 0.54μM NAA plus 0.22 μM BA followed by media containing 4.93 μM IBA either alone or in a combination with 0.22 μM BAThe highest average number of roots/shoot and average root length (em) were achieved on medium contained 4.93 μM IBA plus 0.22 μM BA. The rooted plantlets were successfully acclimatized with 60% survival.

Summary in Arabic.

1