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Micropropagation of Hyoscyamus muticus L. and Datura metel L. by using shoot tips explants [electronic resource].

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Koriech, E. M

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Language: English Summary language: Arabic Description: p.103-110Other title:

الإكثار الدقيق لنباتى السكران المصرى والداتورة ميتل [Added title page title]

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Agricultural research journal, Suez Canal University, 2007 v.7(3) [electronic resource].

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In: Agricultural Research Journal, Suez Canal University 2007 v.7(3)Summary: Shoot tips of Hyoscyamus muticus L and Datura metel L were excised and placed on B5 medium supplemented with BA at 0.0, 0.25 and 0.5 mgl⁻¹ combined with NAA at 0.0, 0.125, 0.25 and 0.5 mgl⁻¹ After 4 weeks, the addition of 0.25 or 0.5 mgl⁻¹BA to B5 medium in the absence or presence of low concentration of NAA (0.125 mgl⁻¹) was the optimal for the production of shoots of H. muticus. The presence of NAA at 0.125 mgl⁻¹ increased both shoot length and callus formation. Also, in D. metel, the addition of 0.25 mgl⁻¹ BA and 0.125 mgl⁻¹ NAA to B5 medium was optimal for increasing number of shoots.

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Shoot tips of Hyoscyamus muticus L and Datura metel L were excised and placed on B5 medium supplemented with BA at 0.0, 0.25 and 0.5 mgl⁻¹ combined with NAA at 0.0, 0.125, 0.25 and 0.5 mgl⁻¹ After 4 weeks, the addition of 0.25 or 0.5 mgl⁻¹BA to B5 medium in the absence or presence of low concentration of NAA (0.125 mgl⁻¹) was the optimal for the production of shoots of H. muticus. The presence of NAA at 0.125 mgl⁻¹ increased both shoot length and callus formation. Also, in D. metel, the addition of 0.25 mgl⁻¹ BA and 0.125 mgl⁻¹ NAA to B5 medium was optimal for increasing number of shoots.

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